ISSN:
1436-2023
Keywords:
Key words GTP-binding protein
;
Carboxyl methylation
;
Isoprenylation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract We examined guanosine triphosphate (GTP)-binding proteins (G-proteins) in synovial tissues obtained from patients with rheumatoid arthritis or osteoarthritis. The results of immunoblot analysis with anti-bovine G-protein βγ-subunit antibody in the rheumatoid synovial tissue were similar to those in the osteoarthritis synovial tissue. On the other hand, the carboxyl methylation activity on G-protein γ-subunit in the rheumatoid synovial tissue was enhanced compared with that in the osteoarthritis synovial tissues: Km and Vm values were 2.6 μM and 10 pmol/mg·min, respectively, for the rheumatoid arthritis, and 4.8 μM and 5.6 pmol/mg·min, respectively, for the osteoarthritis. These results suggest that G-protein-linked signal transduction, in reference to carboxyl methylation of the γ-subunit, is affected in rheumatoid tissues.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s007760070025
Permalink