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  • 1
    Electronic Resource
    Electronic Resource
    A multicopy suppressor gene,MSS10, restoresSTA2 expression inSaccharomyces cerevisiae strains containing theSTA10 repressor gene (1996)
    Springer
    Current genetics 29 (1996), S. 523-529 
    Details
    ISSN: 1432-0983
    Keywords: Glucoamylase ; Starch degradation ; Transcriptional repression/activation ; Yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Transcription of the three unlinked, homologousSTA1-3 glucoamylase-encoding genes, involved in starch degradation bySaccharomyces cerevisiae, was previously shown to be down-regulated by the presence ofSTA10, acting via three upstream repression sequence regions that were identified in theSTA2 promoter. Here we report the cloning and characterization of a putative transcriptional activator gene,MSS10 (multicopy suppressor ofSTA10), which, when present in multiple copies, overcomes STA10 repression. Deletion ofMSS10, located on chromosome XV, resulted in media-specific extinction of glucoamylase synthesis. The nucleotide sequence ofMSS10 is identical to three other genes fromS. cerevisiae identified as:FUP1, a gene that enhances iron-limited growth;PHD2, a gene identified for its ability to induce pseudohyphal growth in diploid cells grown on nitrogen-limited media; andMSN1, a gene encoding a transcriptional activator involved in invertase regulation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02426956
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  • 2
    Electronic Resource
    Electronic Resource
    A multicopy suppressor gene, MSS10, restores STA2 expression in Saccharomyces cerevisiae strains containing the STA10 repressor gene (1996)
    Springer
    Current genetics 29 (1996), S. 523-529 
    Details
    ISSN: 1432-0983
    Keywords: Key words Glucoamylase ; Starch degradation ; Transcriptional repression/activation ; Yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Transcription of the three unlinked, homologous STA1–3 glucoamylase-encoding genes, involved in starch degradation by Saccharomyces cerevisiae, was previously shown to be down-regulated by the presence of STA10, acting via three upstream repression sequence regions that were identified in the STA2 promoter. Here we report the cloning and characterization of a putative transcriptional activator gene, MSS10 (multicopy suppressor of S TA 10), which, when present in multiple copies, overcomes STA10 repression. Deletion of MSS10, located on chromosome XV, resulted in media-specific extinction of glucoamylase synthesis. The nucleotide sequence of MSS10 is identical to three other genes from S. cerevisiae identified as: FUP1, a gene that enhances iron-limited growth; PHD2, a gene identified for its ability to induce pseudohyphal growth in diploid cells grown on nitrogen-limited media; and MSN1, a gene encoding a transcriptional activator involved in invertase regulation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940050081
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Shared control of maltose induction and catabolite repression of the MAL structural genes in Saccharomyces (1994)
    Springer
    Molecular genetics and genomics 243 (1994), S. 622-630 
    Details
    ISSN: 1617-4623
    Keywords: Saccharomyces cerevisiae ; Yeast Catabolite repression ; Gene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Maltose utilization in yeast requires the presence of any one of the five unlinked, homologous MAL loci. Transcription of the two structural genes MALT (permease) and MALS (maltase) is induced by maltose and catabolite-repressed by glucose. MAL6T and MAL6S share a common 5′ intergenic sequence; deletion studies within this sequence revealed a bi-directionally functioning upstream activation sequence (UASM) consisting of four 11bp homologous sites. Activation of these sites by the MALR protein results in the coordinate expression of MAL6T and MAL6S. The basal promoter activates MALS expression to a greater extent than MALT and is located in a region that overlaps UASM. Deletion of several subsites within the UASM has an asymmetric effect on MAL gene expression, having a greater affect on MALT than on MALS. Catabolite repression of MAL6T and MAL6S by glucose is controlled at several levels. Using disruption mutants, the positively acting MAL1R protein was also found to play a role in catabolite repression of MAL6T and MAL6S.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00279571
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  • 4
    Electronic Resource
    Electronic Resource
    Primary structure of the regulatory gene from the MAL6 locus of Saccharomyces carlsbergensis (1988)
    Springer
    Molecular genetics and genomics 213 (1988), S. 56-62 
    Details
    ISSN: 1617-4623
    Keywords: Maltose ; Nucleotide sequence ; Regulatory protein ; DNA binding ; Saccharomyces
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We determined the complete nucleotide sequence of the yeast MAL6R gene from the Saccharomyces carlsbergensis MAL6 locus. The MAL6R gene encodes a trans-acting protein required for the inducible, coordinate expression of the two divergently transcribed structural genes, MAL6T (maltose permease), and MAL6S (maltase) at this locus. The transcription initiation sites for MAL6R were determined by primer extension experiments. The MAL6R gene contains an open reading frame of 473 amino acids with a calculated Mr of 54892. The N-terminus of the deduced protein contains an amino acid sequence isologous to a consensus sequence for cysteine-zinc associated DNA binding fingers found in other fungal DNA binding proteins. The MAL6R gene was mapped to chromosome VIII by using OFAGE (orthagonal field alternating gel electrophoresis) gels and hybridization with specific chromosome and MAL6 probes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00333398
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  • 5
    Electronic Resource
    Electronic Resource
    Structure of the multigene family of MAL loci in Saccharomyces (1989)
    Springer
    Molecular genetics and genomics 217 (1989), S. 60-69 
    Details
    ISSN: 1617-4623
    Keywords: Maltose ; MAL ; Saccharomyces ; Multigene family
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Multigene families are a ubiquitous feature of eukaryotes; however, their presence in Saccharomyces is more limited. The MAL multigene family is comprised of five unlined loci, MAL1, MAL2, MAL3, MAL4 and MAL6, any one of which is sufficient for yeast to metabolize maltose. A cloned MAL6 locus was used as a probe to facilitate the cloning of the other four functional loci as well as two partially active alleles of MAL1. Each locus could be characterized as a cluster of three genes, MALR (regulatory), MALT (maltose transport or permease) and MALS (structural or maltase), encoded by a total of about 7 kb of DNA; however, homologous sequences at each locus extend beyond the coding regions. Our results indicate that there is extensive homology among the MAL loci, especially within their maltase genes. The greatest sequence diversity occurs in their regulatory gene regions. Southern cross analyses of the cloned MAL loci indicate a single duplication of the MAL6R-homologous sequences upstream of the MAL6R gene as well as an extensive duplication of more than 10 kb at the MAL3 locus. The large repeat at the MAL3 locus results in the presence of four copies of MAL3R-homologous sequences and two copies of MAL3T-homologous sequences at that locus. Two naturally occurring inactive alleles of MAL1 show a deletion or divergence of their MALR sequences. The significance of these repeats in the evolution of the MAL multigene family is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00330943
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  • 6
    Electronic Resource
    Electronic Resource
    The S1, S2 and SGA1 ancestral genes for the STA glucoamylase genes all map to chromosome IX in Saccharomyces cerevisiae (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 11 (1995), S. 783-787 
    Details
    ISSN: 0749-503X
    Keywords: Saccharomyces cerevisiae ; genome mapping ; chromosome IX ; genomic rearrangement ; glucoamylase ; SGA1 ; STA ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The polymorphic extracellular glucoamylase-encoding genes STA1 (chr. IV), STA2 (chr. II) and STA3 (chr. XIV), from Saccharomyces cerevisiae var. diastaticus probably evolved by genomic rearrangement of DNA regions (S1, S2 and SGA1) present in S. cerevisiae, and subsequent translocation to unlinked regions of chromosomal regions. S1, encoding a homologue to the threonine/serine-rich domain of STA glucoamylases (GAI-III), mapped to the right arm of chromosome IX. S2, encoding the hydrophobic leader peptide of GAI-III, was also mapped on the right arm of chromosome IX, next to S1, close to DAL81. The SGA1 sporulation-specific, intracellular glucoamylase-encoding gene is located on the left arm of chromosome IX, 32 kb proximal of HIS5.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320110810
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