ISSN:
1471-4159
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
Abstract: A neutral, mannose-rich, concanavalin A (Con A)-binding glycopeptide fraction was obtained by proteolytic digestion of defatted beef brain tissue. Hydrazinolysis followed by gel filtration of the reaction products provided three oligosaccharides. A portion of each oligosaccharide was treated by exhaustive digestion with α-mannosidase. Another portion was subjected to selective acetolysis of Manαl-6Man linkages, providing two fragments that were recovered by gel filtration. The structure of the intact oligosaccharides, as well as the fragments obtained by selective acetolysis and enzymatic treatment, were resolved by gas-liquid chromatographic-mass spectrometric analysis. The structures of the three oligosaccharides were: (a) Manαl-2Manαl-6(Manαl -3)Manαl-6(Manαl-2Manαl-2Manαl 3)Manβ1-4-N-acetylglucosamine (GlcNAc)β -4N- acetylglucosaminitol (GlcOLNAc); (b) Manαl -2Manαl -6(Manαl -3)Manαl-6(Manαl-2Manαl-3)-Manβ1-4GlcNAcβl -4GlcOLNAc; and (c) Manαl -6(Manαl-3) Manαl - 6(Manαl - 3)Manβl -4GlcNAc-βl - 4GlcOLNAc. These structures account for 15–20% of the glycoprotein-carbohydrate of whole beef brain and most of the oligosaccharides that demonstrate a high affinity for Con A. In view of the large number of Con A-binding glycoproteins in brain tissue, it appears that many of these different glycoproteins must contain structurally identical oligosaccharides.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1471-4159.1983.tb04746.x
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