Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Theophylline protects against glycerol-induced acute renal failure: Regulation of heme oxygenase-1 and glutathione in rat kidney (1997)
    Springer
    Clinical and experimental nephrology 1 (1997), S. 204-211 
    Details
    ISSN: 1437-7799
    Keywords: glycerol-induced acute renal failure ; glutathione ; theophylline ; buthionine sulfoximine ; heme oxygenase-1 ; oxidant stress
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Background To investigate the mechanism of glycerol-induced renal injury, we examined kidney levels of the cellular antioxidant glutathione and heme oxygenase-1 (HO-1), the rate-limiting enzyme in heme degradation, after glycerol injection. Methods Male Wistar rats were injected with glycerol to induce acute renal failure. Serum creatinine levels were used as a marker of renal function at 24 hours after glycerol injection. Theophylline and buthionine sulfoximine or vehicle were administered to the rats after glycerol injection, and we determined renal glutathione levels (by biochemical assay) and the levels of HO-1 protein and messenger ribonucleic acid (mRNA; using immunoblot analysis [kidney only]) in various rat organs at 24 hours after glycerol injection. Results Glutathione levels abruptly declined after glycerol injection, reached a minimum at 4 hours, then returned to about two thirds of control levels at 24 hours. HO-1 protein was detected at 4 hours and reached a maximum at 24 hours. The induction of HO-1 protein was observed only in the kidney. HO-1 mRNA was faintly expressed at 2 hours, increased until at least 8 hours, and was not detected 24 hours after the treatment. When theophylline was administered to glycerol-injected rats, renal function improved and glutathione levels increased. In addition, the levels of HO-1 protein decreased, compared with those of glycerol-treated rats not given theophylline. Conclusions These results suggest that theophylline may act by modulating the HO-1 directly or indirectly.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02480696
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Effect of increased distal sodium delivery on organic osmolytes and cell electrolytes in the renal outer medulla (1992)
    Springer
    Pflügers Archiv 422 (1992), S. 233-238 
    Details
    ISSN: 1432-2013
    Keywords: Renal outer medulla ; Cell electrolytes ; Sodium loading ; Cell osmoregulation ; Organic osmolytes ; Glycerophosphorylcholine ; Betaine ; Inositol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Sodium absorption in distal tubule segments was stimulated by increasing the distal delivery via infusion of hypertonic saline. In these animals, and in control rats, electrolyte concentrations in thick ascending limb cells, light and dark cells of the collecting duct in the outer and inner stripe of the outer medulla and in cells of the proximal straight tubule (outer stripe only) were studied. The measurements were performed by electron microprobe analysis of freeze-dried cryosections of the outer medulla. In addition, organic osmolytes (glycerophosphorylcholine, betaine and myo-inositol) were measured by high performance liquid chromatography in cortex and outer medulla. Augmented delivery of sodium chloride to the distal tubule was associated with increased sodium concentrations of thick ascending limb cells both in the outer and inner stripe and of medullary collecting duct light and dark cells in the outer stripe. While the sum of organic osmolyte concentrations was 28% higher in the outer medulla of the salt-loaded animals compared with controls, this value was unchanged in the renal cortex. These findings indicate that the primary event underlying stimulation of sodium absorption along the thick ascending limb during increased distal sodium delivery is enhanced entry of sodium across the apical cell membrane. This would be expected to lead to higher cell sodium concentrations and stimulation of basolateral active Na-K-exchange. The enhanced transport activity of outer medullary tubules may be associated with increased interstitial tonicities and intracellular retention of organic osmolytes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00376207
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...