ISSN:
1573-6830
Keywords:
retina
;
K+ channel
;
MBK1
;
Kv1.1
;
in vitro expression
;
oocyte
;
mouse
;
Xenopus laevis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary 1. The neurons of the retina have electrical properties that are different from those of most of the other neurons of the central nervous system. To identify the voltage-gated ion channels found in the retina, we screened mouse retinal cDNA libraries with oligonucleotide probes homologous to the mammalian K+ channel MBK1 (Kv1.1) and ligated two partial clones to produce a full-length clone with no significant differences from MBK1. 2. Expression of MBK1 mRNA was determined by RNAse protection. MBK1 mRNA was detected in retinal RNA and was also detected in brain, liver, and heart RNAs. 3. We transcribed the full-length clone, injected it into oocytes ofXenopus laevis, and measured the membrane currents 2 to 6 days later. Depolarization from a holding voltage of -90 mV induced a slowly activated outward current with a peak value as large as 20µA. The current inactivated very slowly with a single exponential time course [mean time constant, 6.5 ± 0.4 sec (SEM) for activation voltage of -10 mV]. 4. The outward current was reduced to half-maximal by 0.42 mM tetraethylammonium, 1.1 mM 4-aminopyridine, and 3.2 mM Ba2+ but was not significantly attenuated by Co2+ (1 mM). 5. The reversal potential (measured with tail currents) changed by 53 mV per decade change of [K+] from 1 to 77 mM. 6. The voltage for half-maximal activation of the conductance was -26.6 mV (±1.7 mV), and the voltage required for ane-fold increase in conductance was 6.9 mV (±0.5 mV). 7. Thus, the mRNA for MBK1 found in the mouse retina causes the expression of a voltage-dependent K+ current which has properties suitable for may retinal neurons.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00741449
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