ISSN:
1013-9826
Source:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Topics:
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
The aim of this paper was the HA and β-TCP powers were synthesized by a new wetchemicalmethod using eggshell and phosphoric acid. The biocompatibility of synthesized naturalHA, HA/β-TCP(50:50) and β-TCP derived from eggshell was compared with those of ascommercial chemical powder with mesenchymal stem cells derived from human bone marrow.Development of crystalline phases of the mixtures was studied as functions of mixing ratio andtemperature using X-ray diffractometer. The morphological characteristics of the calcinedeggshell and synthesized powders were examined by scaning electron microscopy. The in-vitrocytotoxicity and cell attachment of sintered disks were examined using human bone marrowderivedmultipotent stem cells(hBMSCs). Cell response was characterized by MTT assay ,Alkaline phosphatase stain and RT-PCR analysis. Pure HA was synthesized in the mixing ratio of1:1.1 wt% at 900℃ for 1h. the crystallization of HA was started at 800℃ in the 1:1.1 mixingratio, ant the HA phase was continued up to the high temperatures. In the ratio of 1:1.3 and1:1.5 wt%, β-TCP was effectively synthesized at 900℃. In the 1:1.5 ratio, β-TCP phase wasdetected at 700℃, and complete crystallized β-TCP was observed above 900℃. At the highertemperature than 1000℃, the β-TCP was gradually decreased and α-TCP was observed. The HAand β-TCP disk does not exert cytotoxic effect on the hBMSCs undergoing osteoblasticdifferentiation. In addition, the hBMSCs are adhered on the surface of synthesized natural HAand β-TCP disk as successfully as on the culture plate or as commercial chemical HA and β-TCPdisk. The hBMSCs adhered on either synthesized natural HA, β-TCP or as commercial chemicalHA, β-TCP disk displays undistinguishable actin arrangement and cellular phenotypes, indicatingthat synthesized natural HA, β-TCP does not disrupt normal cellular responses. Analysis ofdifferentiation of the hBMSCs cultured on culture plate, synthesized natural HA, β-TCP and ascommercial chemichal HA, β-TCP disk shows that three matrices are able to support osteoblasticdifferentiation of the hBMSCs as accessed by alkaline phosphatase staining
Type of Medium:
Electronic Resource
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/01/52/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.330-332.23.pdf
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