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  • 1
    Electronic Resource
    Electronic Resource
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Plant Physiology 32 (1981), S. 267-289 
    ISSN: 0066-4294
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Plant, cell & environment 12 (1989), S. 0 
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract. Rapid-cooling pulses to non-stressful temperatures cause strong, transient depolarizations in cortical cells of cucumber roots. The amplitudes of these electrical responses are graded according to the rate and amplitude of the cooling pulse. Such graded potentials are typical of sensory processes and indicate that plants possess the ability to sense temperature change. La3+, a blocker of Ca2+ channels, and ethylene glycol bis-(β-aminoethyl ether) N,N,N′,N′-acetic acid (EGTA), a Ca2+ chelator, inhibit the electrical responses elicited by rapid-cooling pulses. High external [Ca2+] enhances them. These results indicate the involvement of a plasma membrane-associated Ca2+ channel in the process of temperature sensing by plants. Calmodulin antagonists prolong the repolarization phase of the electrical responses, suggesting a role for calmodulin in the recovery from stimulation.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 218 (1968), S. 357-357 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The proponents of the sorption theory claim that most of the water in the cytoplasm of living cells is maintained in an ice-like state by its close association with protein and that this reduces the ionic solubilities. The rest of the water is said to be freely accessible to substances in the ...
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 14 (1961), S. 312-313 
    ISSN: 0001-5520
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Geosciences
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Planta 102 (1972), S. 215-227 
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Electrical coupling between adjacent cells of Elodea canadensis has been demonstrated using a microelectrode technique in which the membrane potentials were recorded during the passage of a current pulse from the vacuole of one cell to the external solution. The changes in membrane potential resulting from the passage of the current may be simulated by an equivalent circuit in which the tonoplast:plasmalemma:plasmodesmata resistances are in the ratio 1.0:5.6:2.2. On this basis, the specific resistances are 3.1 kΩ cm2 for the plasmalemma, 1.0 kΩ cm2 for the tonoplast and 0.051 kΩ cm2 for the junction between the cells. Although the plasmodesmata permit the passage of current, it is estimated that they have a resistance about 60 times higher than would be the case if they were completely open channels. Electrical coupling has also been demonstrated between parenchymal cells in oat coleoptiles and between cortical cells in maize roots. The significance of these findings is discussed in relation to the symplastic transport of ions and other small molecules and in relation to the quantitative measurement of membrane resistance in multicellular tissue.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    The journal of membrane biology 2 (1970), S. 59-70 
    ISSN: 1432-1424
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary The effects of bicarbonate ions on the membrane potential and resistance ofNitella translucens are shown to be primarily due to the change in pH produced by the bicarbonate acting as a buffer, and not due to the presence of an electrogenic anion pump. The mechanism by which pH affects the membrane potential is discussed in the context of recent work by other authors on this effect.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1615-6102
    Keywords: Nuclear magnetic resonance ; Magnetic resonance imaging ; Pisum sativum L. ; Sucrose ; Water suppression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Developing pea (Pisum sativum L.) seeds were chosen to evaluate the performance of various nuclear magnetic resonance (NMR) and magnetic resonance imaging (MRI) methods of detecting sucrose in plants. The methods included chemical shift selective imaging (CHESS), heteronuclear correlation via13C-1H coupling (HMQC), and homonuclear correlation via1H-1H coupling (DQF). The same experiments were also performed on sucrose phantom samples to evaluate the methods in the absence of the line broadening observed in plant systems. Using the spin echo technique for multi-slice imaging, we could discern the detailed internal structure of the intact seed with a resolution of tens of microns. The proton spin-lattice relaxation time and linewidth as a function of the age of the seed were measured to optimize the efficiency of the NMR and MR experiments. The age-dependent changes in these NMR parameters are consistent with the accumulation of insoluble starch as age increases. Both the NMR and MRI results are in accord with the results of chemical analysis, which reveal that the sucrose concentration is higher in the embryo than in the seed coat, and glucose is at low concentration throughout the seed. Of the three methods for proton observation, the enhanced version of the CHESS approach (CD-CHESS) provides the best combination of sucrose detection and water suppression. Direct observation of13C is preferable to indirect detection using HMQC because of water signal bleed-through in samples with large (〉200 Hz) linewidths.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 128 (1985), S. 167-172 
    ISSN: 1615-6102
    Keywords: Intercellular transport ; Plasmodesmata ; Setcreasea purpurea ; Symplastic transport
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Previously reported studies of cell ultrastructure and molecular probe passage in immature staminal hairs were extended to kinetic studies. The rate of transport of carboxyfluorescein into the cytoplasm in individual cells was monitored with a video analyzer and transport coefficients were determined. Carboxyfluorescein was found to traverse 5 cells in less than 5 minutes. The values of transport coefficients differed between cells and this was taken to mean that some cells are more closely coupled than others.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Plant cell, tissue and organ culture 23 (1990), S. 107-114 
    ISSN: 1573-5044
    Keywords: electrofusion ; grape ; polyethylene glycol ; protoplasts ; vacuoles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cultured grape cells accumulate anthocyanins in vacuoles rather than secreting them into the nutrient medium. Therefore, grape cells that contain tonoplast segments in their plasmalemma should be capable of excreting anthocyanins rather than sequestering them in their vacuoles. In initial attempts to construct such ‘novel’ cells, small vacuoles were fused with the plasmalemma of cultured plant cells. Protoplasts were isolated from grape calluses that produce and accumulate anthocyanins. Small vacuoles were formed by gently rupturing vacuoles isolated from grape protoplasts. Although small vacuoles and protoplasts became aligned in an AC field, the tonoplast and plasmalemma did not readily fuse when subjected to 3 DC pulses of 1200 V cm−1 for 50 μs each. Changes in the intensity, number and/or duration of the DC pulses had no effect on the fusion process. When 1.0% polyethylene glycol was added to the electrofusion buffer, however, small vacuoles and protoplasts fused within a few minutes after the DC pulses were applied. These ‘novel’ grape cells remained viable for several hours.
    Type of Medium: Electronic Resource
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