ISSN:
1435-604X
Keywords:
Fluorescence
;
Time-resolved confocal microscopy
;
Porphyrins
;
Fluorescence polarization
;
Photodynamic therapy
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
,
Physics
,
Technology
Notes:
Abstract The application of a novel time-resolved confocal fluorescence microspectrometer to studies of the distribution and speciation of porphyrin photosensitizers in rat C6 cerebral glioma cells is described. The instrument combines a mode-locked argon ion laser excitation source with time-correlated single photon counting fluorescence detection and has sub-micron spatial and sub-nanosecond temporal resolution. The porphyrins studied were haematoporphyrin derivative (HpD), haematoporphyrin IX (HP), porphyrinc (Pc) and the tetrakiscarborane carboxylate ester of 2,4-(α,β-dihydroxyethyl) deuteroporphyrin IX (BOPP). From the heterogeneous emission observed in vitro, assignments and spatial location of various porphyrin species are proposed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02134914
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