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  • 1
    Electronic Resource
    Electronic Resource
    Interaction of H2O, CH3OH, (CH3)2O, CH3CN, and Pyridine with the Superacid Perfluorosulfonic Membrane Nafion: An IR and Raman Study (1995)
    s.l. : American Chemical Society
    The @journal of physical chemistry 〈Washington, DC〉 99 (1995), S. 11937-11951 
    Details
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/j100031a023
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  • 2
    Electronic Resource
    Electronic Resource
    Spectroscopic Studies (UV-vis and FTIR) of CO and Ethene Molecular Complexes and of Ethene Oligomerization on .alpha.-Cr2O3 Surfaces (1994)
    s.l. : American Chemical Society
    Langmuir 10 (1994), S. 3094-3104 
    Details
    ISSN: 1520-5827
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/la00021a037
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  • 3
    Electronic Resource
    Electronic Resource
    Cyclic AMP phosphodiesterase activity in human gingival carcinoma (2004)
    Oxford, UK : Munksgaard International Publishers
    Journal of oral pathology & medicine 33 (2004), S. 0 
    Details
    ISSN: 1600-0714
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background:  The phosphodiesterases (PDEs) are responsible for the hydrolysis of the second messengers, cyclic AMP (cAMP) and cyclic GMP (cGMP), to their corresponding monophosphates with a fundamental role in the transduction of the intracellular signals. At least 11 different enzymatic isoforms have been identified, which are listed according to their specificity or affinity for the substratum, identity of the amino acid sequence, cofactor, and inhibitor sensitivity. Variations in PDE activity have been found in different pathologies, and they have also been correlated to different pathological e/o physiological mechanisms, such as cellular differentiation, apoptosis, and tumor invasivity.Objectives:  In this study, we have evaluated cAMP PDE activity in patients with carcinoma of the gingiva, with the purpose of correlating differences in its development and progression. The same enzymatic activity has been used to evaluate differences between patients with lymph node involvement (group N+), and patients without lymph node involvement (N−).Materials and Methods:  The analysis of PDE activity and the cAMP assay was performed by reverse-phase HPLC on samples of fresh or frozen gingival tissues. Analysis of cAMP was confirmed with the enzyme-linked immunoabsorption assay (EIA).Results and Conclusions:  The differences between control and N– groups (P = 0.0433), and between control and N+ groups (P = 0.0156) were statistically significant. PDE3A was also evaluated immunohistochemically in lymph-node negative and lymph-node positive cases. The differences between the two groups were statistically significant (P = 0.0397).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.0904-2512.2004.00092.x
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  • 4
    Electronic Resource
    Electronic Resource
    Cyclic guanosine monophosphate phosphodiesterase activity in human gingival carcinoma (2003)
    Oxford, UK : Munksgaard International Publishers
    Journal of oral pathology & medicine 32 (2003), S. 0 
    Details
    ISSN: 1600-0714
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background:  Cyclic guanosine monophosphate (cGMP) is an essential second messenger metabolized by phosphodiesterases (PDEs).Objectives:  We looked for a possible correlation of PDE activities in human oral squamous cell carcinoma (OSCC) with and without lymph node metastases.Materials and methods:  The analysis of phosphodiesterase activity and the cGMP assay were done by reverse-phase HPLC on samples of fresh or frozen gingival tissues. Analysis of cGMP was confirmed with the enzyme-linked immunoabsorption assay.Results and conclusions:  cGMP PDE activity was 34.92 ± 7.17 SD, 12.89 ± 4.43 SD, and 35.88 ± 8.76 SD (nmols/mg of protein), respectively, in controls, samples without lymph node involvement (N−), and specimens with lymph node metastases (N+). cGMP values were 1.97 ± 0.63 SD, 3.30 ± 1.47 SD, and 3.49 ± 1.47 SD (nmols/mg of protein). Our data support the hypothesis of a role for cGMP and PDE in the progression of OSCC.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1034/j.1600-0714.2003.00083.x
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  • 5
    Electronic Resource
    Electronic Resource
    Longitudinal monitoring of subgingival colonization by Actinobacillus actinomycetemcomitans, and crevicular alkaline phosphatase and aspartate aminotransferase activities around orthodontically treated teeth (2004)
    Oxford, UK : Munksgaard International Publishers
    Journal of clinical periodontology 31 (2004), S. 0 
    Details
    ISSN: 1600-051X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Objectives: During orthodontic treatment, changes in subgingival plaque colonization and tissue inflammation and remodelling have been described. This study uses a longitudinal design to examine subgingival colonization of Actinobacillus actinomycetemcomitans (Aa) and alkaline phosphatase (ALP) and aspartate aminotransferase (AST) activities in gingival crevicular fluid (GCF) in order to assess whether these parameters have potential as biomarkers of tissue responses to orthodontic tooth movement in humans.Materials & Methods: Twenty-one patients (ages: 11.2–22.5; mean 17.1±3.3 years) participated in the study. An upper canine from each patient undergoing treatment for distal movement served as the test tooth (DC), and its contralateral (CC) and antagonist (AC) canines were used as controls. The CC was included in the orthodontic appliance, but was not subjected to the orthodontic force; the AC was free from any orthodontic appliance. The subgingival plaque and GCF around the experimental teeth was harvested from both mesial and distal tooth sites immediately before appliance activation and on day 28. Clinical gingival condition was evaluated at the baseline and at the end of the experimental period. Aa colonization was determined by culture methods, while ALP and AST activities were evaluated spectrophotometrically.Results: Throughout the study, the clinical conditions worsened in both the DCs and the CCs as compared with the baseline, whereas no significant differences were found between the DCs and the CCs, or between mesial and distal sites of each of these teeth on day 28. In the ACs, clinical parameters remained at baseline levels throughout the study. Similar results were found for Aa colonization, which increased significantly on day 28 in the DC and CC groups. On day 28, ALP and AST activities were significantly elevated in all sites from the DC and CC groups as compared with the ACs, where, conversely, enzymatic activities remained at the baseline levels. However, ALP activity in the DC group was significantly greater than in the CCs at mesial (tension) sites on day 28, while AST activity in the DCs was significantly elevated as compared with the CC group at the distal (compression) sites. Greater ALP activity in the DC group was observed at the tension sites compared with the compression sites on day 28.Conclusions: Our results suggest that Aa subgingival colonization, and ALP and AST activities in GCF reflect the tissue responses that occur in the periodontium during orthodontic treatment.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.0303-6979.2004.00450.x
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  • 6
    Electronic Resource
    Electronic Resource
    Well defined CuI(NO), CuI(NO)2 and CuII(NO)X (X = O− and/or NO 2 − ) complexes in CuI-ZSMS prepared by interaction of H-ZSM5 with gaseous CuCl (1992)
    Springer
    Catalysis letters 13 (1992), S. 39-44 
    Details
    ISSN: 1572-879X
    Keywords: Zeolites ; H-ZSM5 ; Cu-ZSM5 ; NO ; Nitrosyl complexes ; NO decomposition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract In this note an exchange procedure of the acidic protons of H-ZSM5 by CuI ions through reaction with CuCl in the gas phase is described. In the so obtained CuI-ZSM5 exchanged zeolite the CuI ions are in well defined configuration and form with NO mono and di-nitrosyl complexes of high structural and spectroscopic quality. The CuI(NO)2 species are transformed at RT into CuII(NO)X (X=O− and/or NO 2 − ) species which could represent an intermediate in NO decomposition.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00770945
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  • 7
    Electronic Resource
    Electronic Resource
    Reduced human lymphocyte blastogenesis and enhancement of adenosine triphosphate (ATP) by L-carnitine (1994)
    Springer
    Molecular and cellular biochemistry 131 (1994), S. 1-8 
    Details
    ISSN: 1573-4919
    Keywords: lymphocytes ; PHA ; DNA ; blastogenesis ; ATP ; L-Carnitine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Carnitine is associated with lipid synthesis and its deficiency may lead to cardiomegaly with parenchimal lipid in the heart, kidney and liver. In our study we found that pretreatment of peripheral blood mononuclear cells (PBMC) with serial dilutions of L-Carnitine (100μg/ml-1 pg/ml) inhibits, in a dose-dependent manner, lymphocyte DNA synthesis stimulated with PHA (20 μg/ml). L-Carnitine did not have any effect on resting PBMC. The maximum inhibition was found at 10 μg/ml of L-Carnitine. Moreover, in a time-course study and using an enzymatic analysis (ATP monitoring reagent), L-Carnitine enhanced ATP production on PBMC treated and untreated with PHA, reaching a maximum effect at 30 min incubation. In another set of experiments PBMC were treated with L-Carnitine alone and in combination with PHA, and the percent of receptors CD3, CD4, and CD8 were calculated with flow cytometry. After the cell incubation with L-Carnitine, the percent of all receptors studied did not change compared to L-Carnitine-untreated cells (controls). These data suggest that L-Carnitine inhibits, in a dose-dependent manner, lymphocyte blastogenesis induced by PHA, probably through the enhancement of ATP synthesis, which is considered an inhibitor of phospholipase C activity and a suppressor in lymphocyte cultures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01075718
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  • 8
    Electronic Resource
    Electronic Resource
    Heterocycles oligomerization in acidic zeolites: a UV-visible and IR study (1999)
    Springer
    Topics in catalysis 8 (1999), S. 279-292 
    Details
    ISSN: 1572-9028
    Keywords: acid zeolites ; heterocycles ; oligomerization ; UV-Vis ; FTIR
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract FTIR spectroscopy shows that the first step of the interaction of pyrrole, furan and thiophene with H- $$\beta $$ and H-ZSM-5 zeolites is the formation of hydrogen bonded (precursor) species involving the Brønsted acid sites of the zeolite and the $$\pi $$ -electron system of the heterocyclic molecule. The precursors are then protonated to give monomeric BH+ (where B = C4H4NH, C4H4O or C4H4S) species which, in the presence of excess B, can further react to form entrapped coloured BnH+ oligomeric species. Characterization by means of in situ UV-Vis reflectance spectroscopy shows that the oligomeric products contain up to six conjugated double bonds and that they have carbocationic nature. However, the precise structure of the responsible species (particularly those absorbing in the visible) cannot be completely elucidated. The carbocationic character of the oligomers is also documented by the changes induced in the electronic spectra by adsorption of NH3, i.e., of a base capable of extracting H+ ions from BnH+ to give NH 4 + and Bn. The role of intermolecular hydrogen transfer in the intrazeolitic oligomerization process is also evidenced and discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1019137900710
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