ISSN:
1432-203X
Keywords:
Key words: Protoplasts –Hordeum vulgare L. – nurse culture – plant regeneration – primary callus
;
Abbreviations: 2,4-D: 2,4-dichlorophenoxyacetic acid, BAP: 6-benzylaminopurine
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary. Primary callus of barley (Hordeum vulgare L.) derived from scutella (cv. ‘Igri’) was used for protoplast isolation and plant regeneration. The protoplasts were embedded in agarose and cultured with nurse cells. The plating efficiency varied from 0.1% to 0.7%. Shoots regenerated from the developing callus. Plantlets were transferred to soil and cultivated in the greenhouse three to five months after protoplast isolation. All plants were normal in morphology, and most of them flowered and set seeds.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01890854
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