ISSN:
1399-0047
Source:
Crystallography Journals Online : IUCR Backfile Archive 1948-2001
Topics:
Chemistry and Pharmacology
,
Geosciences
,
Physics
Notes:
Crystals of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase from Pseudomonas mevalonii have been grown by vapor diffusion in hanging drops at pH 6.7 using ammonium sulfate as the precipitant. Serial dilution seeding and manipulation of glycerol concentration were both used to obtain crystals larger than 1.0 mm. The crystals are cubic, space group I4132, with a = 229.4 Å. A Vm value of 2.71 Å3 Da−l indicates 96 molecules per unit cell with two molecules in the asymmetric unit. These crystals diffract to 2.8 Å with conventional X-ray sources, and beyond 2.4 Å with synchrotron radiation.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1107/S0907444994009819
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