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  • 1
    Electronic Resource
    Electronic Resource
    RAPD and AFLP tagging and mapping of Beta (B) and Beta modifier (MoB), two genes which influence β-carotene accumulation in fruit of tomato (Lycopersicon esculentum Mill.) (2000)
    Springer
    Theoretical and applied genetics 100 (2000), S. 368-375 
    Details
    ISSN: 1432-2242
    Keywords: Key words Molecular marker ; Bulk segregant analysis ; Near-isogenic lines ; Lycopersicon cheesmanii ; Lycopersicon hirsutum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The Beta (B) locus in tomato (Lycopersicon esculentum) increases fruit β-carotene content at the expense of lycopene, resulting in orange-pigmented fruit. Expression of B is influenced by the beta-modifier (Mo B ) gene which segregates independently of B. RAPD and AFLP analyses were performed using near isogenic lines (NILs) unique for B and bulked segregant analysis (BSA) of a L. esculentum×L. cheesmanii-derived F2 population segregating for B. Using 1018 random primers for RAPD analysis and 64 primer pairs for AFLP analysis, we identified polymorphic products which distinguished the NILs and the two bulked DNA samples constructed for BSA. A single 100 bp AFLP amplification product (E-ACA/M-CTG100) which distinguished the NILs cosegregated with Mo B and was demonstrated to be tightly linked to the locus. E-ACA/M-CTG100 exhibited a recombination frequency of 1.7% in the F2 progeny derived from an initial cross between the isolines. The Mo B locus was mapped to the long arm of chromosome 6. Two RAPD products (OPAR181100 and UBC792830) of 1100 bp and 830 bp, respectively, were polymorphic between orange- and red-fruited bulks constructed from F2 individuals in the L. esculentum and L. cheesmanii mating series. OPAR181100 and UBC792830 displayed recombination frequencies of 4.2% and 7.6%, respectively, in F2 progeny. The B-linked OPAR181100 marker was also mapped to the long arm of chromosome 6, proximal to Mo B , and revealed linkage between B and Mo B .
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050048
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  • 2
    Electronic Resource
    Electronic Resource
    Identification of molecular markers associated with leptine production in a population of Solanum chacoense Bitter (1999)
    Springer
    Theoretical and applied genetics 98 (1999), S. 39-46 
    Details
    ISSN: 1432-2242
    Keywords: Key words Glycoalkaloids ; Potato ; Metabolic pathways ; RAPD ; Leptine ; Insect resistance ; Solanum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract   Solanum chacoense Bitter, a wild relative of the cultivated potato, produces several glycoalkaloids, including solanine, chaconine, and the leptines. The foliar-specific leptine glycoalkaloids are believed to confer resistance to the Colorado Potato Beetle (CPB). Using two bulked DNA samples composed of high- and low-percent leptine individuals from a segregating F1 population of S. chacoense, we have identified two molecular markers that are closely linked to high percent solanine+chaconine and, conversely, to nil/low percent leptine. One of these, a 1,500-bp RAPD product (UBC370-1500), had a recombination value of 3% in the F1 progeny, indicating tight linkage. UBC370-1500 mapped to the end of the short arm of potato chromosome 1, in the region of a previously mapped major QTL for solanidine, from a S. tuberosum (solanidine)×S. berthaultii (solasodine) cross. Taken together, these results suggest that either (1) a major locus determining solanidine accumulation in Solanum spp. is on chromosome 1 in the region defined by the RFLP markers TG24, CT197, and CT233, or (2) this region of chromosome 1 may harbor two or more important genes which determine accumulation of steroidal aglycones. These findings are important for the genetics of leptine (as well as other glycoalkaloid) accumulation and for the development of CPB-resistant potato varieties.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051037
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    Paper (German National Licenses)
    Fulltext
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