ISSN:
1432-0991
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract The relationship between sporulation temperature and spore killing temperature is described.Bacillus subtilis YB886, grown and sporulated at 25°, 30°, 37°, and 45°C, produced spores having D90 values of 63.5, 76.3, 89.0, and 106 min respectively. In addition, the vegetative cells of this strain also demonstrated resistance to heat killing when grown at elevated temperatures (D50 of 26.6, 32.5, 39.0, and 〉50 min for cells grown at 25°, 30°, 37°, and 45°C). A transposon-generated mutant of strain YB886, designated as BUL786, which is missing a heat shock-induced protein (97 kDa) (Qoronfleh MW and Streips UN, BBRC, 138:526–532, 1986 and FEMS 1987), was tested for thermotolerance under similar conditions. The cells failed to respond to growth at high temperature by producing heat-resistant spores or vegetative cells. For strain BUL786 the D90 of spores generated at 20°, 25°, 30°, 37°, and 45°C was 9.4, 11.3, 12.8, 14.1, and 20 min, respectively. Similarly, the D50 of vegetative cells was 15, 16.8, 17.8, 19.0, and 22.3 min when the cells were grown at 20°, 25°, 30°, 37°, and 45°C. Also, sporulation of YB886 cells in the presence of cadmium chloride increased the D90 values for the resulting spores (5µM CdCl2 resulted in a D90 of 160 min). Strain BUL786 failed to produce spores with any elevated D90 when grown in the presence of CdCl2.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02092164
Permalink
