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  • 1
    ISSN: 1432-2242
    Schlagwort(e): Hordeum X Triticum-hybrids ; Somatic embryogenesis ; Regeneration ; Inflorescence ; Chromosome doubling
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Embryogenic callus cultures were obtained by culturing young inflorescence tissues of Hordeum vulgare cv. ‘PF51811’ (2x)XTriticum aestivum cv. ‘Chinese Spring’ (6x) hybrids on 2,4-D-containing N6 medium. After subculture for about 10 months the calli retained a high potentiality for somatic embryogenesis and plant regeneration. Of about 300 regenerated plants, approximately 100 were transplanted to potting soil. Eight embryoids and three regenerated plants examined had 28 chromosomes identical to the original hybrid plants, while one regenerated plant was found to be a mixploid composed of cells with 28 and 56 chromosomes. The possibility for obtaining amphiploid hybrids through tissue culture is discussed.
    Materialart: Digitale Medien
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    Theoretical and applied genetics 55 (1979), S. 193-197 
    ISSN: 1432-2242
    Schlagwort(e): Albino pollen plantlet ; Anther culture ; Plastid ribosomal RNA ; Fraction I protein
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The components of soluble protein and ribosomal RNA in green and albino pollen plantlets of rice were studied by means of polyacrylamide gel electrophoresis. The results were as follows: (1) Soluble protein: the soluble proteins in green pollen plantlets may be fractionated into 15 bands of varying intensities in which the highest content and the most prominent one is band 3 (fraction I protein). Band 3, however, is nearly absent in albino pollen plantlets. (2) Ribosomal RNA (rRNA): rRNA of high molecular weight in green pollen plantlets may be fractionated into 4 bands, i.e. 25S RNA and 18S RNA in cytoplasmic ribosomes, and 23S RNA and 16S RNA in plastid ribosomes. Little or no 23S RNA and 16S RNA, however, is found in albino pollen plantlets. Together with the evidence obtained by other workers, it is suggested that albino forms of pollen plantlets is caused by the impairment of DNA.
    Materialart: Digitale Medien
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  • 3
    Digitale Medien
    Digitale Medien
    Springer
    Plant cell reports 8 (1989), S. 313-316 
    ISSN: 1432-203X
    Schlagwort(e): Zea mays L. ; supersweet corn ; protoplasts ; haploids ; plant regeneration
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Plants were regenerated from maize (Zea mays L.) protoplasts isolated from embryogenic cell suspensions. The donor maize suspension cultures were established from friable callus initiated from microspores of a commercial supersweet hybrid (sh2sh2). The frequency of cell colony formation was higher when protoplasts were cultured on feeder layers of maize cells as compared with a liquid thin layer method. It was demonstrated that haploid and dihaploid soil-grown plants can be regenerated from maize protoplasts isolated from haploid cell cultures.
    Materialart: Digitale Medien
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  • 4
    Digitale Medien
    Digitale Medien
    Springer
    Journal of paleolimnology 17 (1997), S. 9-21 
    ISSN: 1573-0417
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Geologie und Paläontologie
    Notizen: Abstract Glacial Lake Hind was a 4000 km2 ice-marginal lake which formed in southwestern Manitoba during the last deglaciation. It received meltwater from western Manitoba, Saskatchewan, and North Dakota via at least 10 channels, and discharged into glacial Lake Agassiz through the Pembina Spillway. During the early stage of deglaciation in southwestern Manitoba, part of the glacial Lake Hind basin was occupied by glacial Lake Souris which extended into the area from North Dakota. Sediments in the Lake Hind basin consist of deltaic gravels, lacustrine sand, and clayey silt. Much of the uppermost lacustrine sand in the central part of the basin has been reworked into aeolian dunes. No beaches have been recognized in the basin. Around the margins, clayey silt occurs up to a modern elevation of 457 m, and fluvio-deltaic gravels occur at 434–462 m. There are a total of 12 deltas, which can be divided into 3 groups based on elevation of their surfaces: (1) above 450 m along the eastern edge of the basin and in the narrow southern end; (2) between 450 and 442 m at the western edge of the basin; and (3) below 442 m. The earliest stage of glacial Lake Hind began shortly after 12 ka, as a small lake formed between the Souris and Red River lobes in southwestern Manitoba. Two deltas at an elevation of 450 were formed in this lake. At the same time, the Souris Lobe retreated far enough to allow glacial Lake Souris to expand farther north along the western side of the basin from North Dakota into what was to become glacial Lake Hind. Three deltas were built at an elevation above 460 m in the Canadian part of this proglacial lake. Continued ice retreat allowed the merger of glacial Lake Souris with the interlobate glacial Lake Hind to the east. Subsequent erosion of the outlet to the Pembina Spillway allowed waters in the glacial Lake Hind basin to become isolated from glacial Lake Souris, and a new level of glacial Lake Hind was established at 442 m, with 5 deltas built at this level by meltwater runoff from the west. Next, a catastrophic flood from the Moose Mountain uplands in southeastern Saskatchewan flowed through the Souris River valley to glacial Lake Souris, spilling into Lake Hind and depositing another delta. This resulted in further incision of the outlet (Pembina Spillway). A second flood through the Souris Spillway from glacial Lake Regina further eroded the outlet; most of glacial Lake Hind was drained at this time except for the deeper northern part. Coarse gravel was deposited by this flood, which differs from previous flood gravel because it is massive and contains less shale.
    Materialart: Digitale Medien
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  • 5
    Digitale Medien
    Digitale Medien
    Springer
    Plant cell, tissue and organ culture 5 (1986), S. 175-178 
    ISSN: 1573-5044
    Schlagwort(e): cereals ; somatic embryogenesis ; tissue culture ; Coix lacryma-jobi
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Callus was obtained from segments of immature inflorescence of Coix lacryma-jobi cultured on N6 medium containing 1–2 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) and 3–5% sucrose. Plantlets were regenerated when embryogenic calluses were transferred onto MS medium with 0.5 mg/l kinetin and 0.01 mg/l naphthaleneacetic acid (NAA). Regenerated plants had the diploid chromosome number (2n=20).
    Materialart: Digitale Medien
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