ISSN:
1615-6102
Keywords:
Cell surface
;
Flagella
;
Lectins
;
Monoclonal antibodies
;
Phytophthora cinnamomi
;
Zoospore encystment
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Only two of a number of macromolecules that bind to the surface of zoospores of the dieback fungus,Phytophthora cinnamomi, induce encystment when added to a suspension of actively swimming zoospores. One, the lectin Concanavalin A (ConA), binds to the entire surface of the zoospores including the surface of both flagella. Within 10 minutes more than 70% of the cells have encysted in the presence of 5 μg/ml ConA. This encystment is inhibited by preincubation of the lectin with its hapten sugar, α-methyl-D-mannoside. The other effective molecule, a monoclonal antibody designated Zf-1, is one of 35 that have been raised to components on the surface of zoospores and cysts ofP. cinnamomi. The antigen for Zf-1 occurs only on the surface of the two flagella. Purified Zf-1 at 15 μg/ml causes encystment of 75% of the zoospores in 13minutes. To show that the induction of encystment by these two probes is not due simply to the presence of protein either in solution or bound to the zoospore a number of other proteins were tested, including other antibodies that bind to the zoospore surface. None of these other molecules caused encystment even at concentrations greater than 200 μg/ml. The results are consistent with the surface components that bind ConA and Zf-1 being involved in the critical step of triggering encystment at the surface of a potential host during infection.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01304632
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