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  • 1
    Electronic Resource
    Electronic Resource
    On-line amperometric assay of glucose, L-glutamate, and acetylcholine using microdialysis probes and immobilized enzyme reactors (1995)
    Weinheim : Wiley-Blackwell
    Electroanalysis 7 (1995), S. 1114-1117 
    Details
    ISSN: 1040-0397
    Keywords: Amperometric detection ; Microdialysis probe ; Immobilized enzyme reactor ; In vivo monitoring ; Glucose ; L-Glutamate ; Acetylcholine ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A highly selective on-line, real-time monitoring system is proposed for amperometric assay of glucose, L-glutamate, and acetylcholine. The system includes a microdialysis probe, immobilized enzyme reactor, and poly(1,2-diaminobenzene)-coated platinum electrode. The analyte in the dialysate from the microdialysis probe is enzymatically converted to produce hydrogen peroxide. The hydrogen peroxide is detected selectively at a poly(l,2-diaminobenzene)-coated platinum electrode, without any interference from oxidizable species and proteins. The present method can be successfully applied to in vitro assay of glucose and in vivo monitoring of glucose in rat brains. However, the sensitivity is not sufficient for in vivo monitoring of trace amounts of L-glutamate and acetylcholine in rat brains.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elan.1140071203
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Microdialysis fiber enzyme electrode involving amplification by substrate recycling (1997)
    Weinheim : Wiley-Blackwell
    Electroanalysis 9 (1997), S. 950-951 
    Details
    ISSN: 1040-0397
    Keywords: Mikrodialysis fiber electrode ; Microenzyme-electrod ; NAD+/NADH sensor ; Substrate recycling ; Amplification ; Size-exclusion film ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A microdialysis fiber electrode involving amplification for the NAD+/NADH couple is described. The electrode contains glucose-6-phosphate dehydrogenase, lactate dehydrogenase and lactate oxidase held stationary within its cavity, with a platinum fiber coated with poly(1,2-diaminobenzene) film as a working electrode and a silver/silver chloride fiber as a reference electrode. Both NAD+ and NADH are recycled enzymatically in the presence of an excess of glucose-6-phosphate and pyruvate in the measuring buffer solution. As a result, a large amount of L-lactate produced in the electrode cavity is converted to pyruvate to produce hydrogen peroxide, which can be detected ampero metrically at the platinum fiber with a size-exclusion film. Both NAD+ and NADH were detected with a 25-fold increase in sensitivity compared with the unamplified responses. The detection limit was 1 × 10-7 M.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elan.1140091217
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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