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  • 1
    Electronic Resource
    Electronic Resource
    Vacuolating Cytotoxin A is Associated with Increased Thrombin Generation in Gastric Mucosa (2005)
    Oxford, UK : Blackwell Science Ltd
    Helicobacter 10 (2005), S. 0 
    Details
    ISSN: 1523-5378
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background.  Activation of the coagulation system is a critical response for both the repair of tissue injury and the host defense against microbial pathogens. Activation of the coagulation cascade culminates with the generation of thrombin. In vitro studies have shown that thrombin protects gastric epithelial cells from injury. The present study was undertaken to assess in vivo the relationship between gastric intramucosal generation of thrombin and Helicobacter pylori infection.Materials and methods.  This study comprised 59 patients with gastroduodenal disorders. There were 27 patients with H. pylori infection (Hp+), 14 without it (Hp–), and 18 patients with cured H. pylori infection (Hp c). The gastric intramucosal concentrations of thrombin–antithrombin complex (TAT), epidermal growth factor (EGF), prostaglandin E2 (PGE2), and vacuolating cytotoxin A (VacA) were measured by specific immunoassays.Results.  The level of TAT was significantly increased in patients with Hp+ compared to Hp– and Hp c. The levels of TAT, EGF and PGE2 were higher in VacA (+) patients than in those with VacA (–). VacA induced significant expression of tissue factor in gastric epithelial cells in vitro. The gastric intramucosal level of VacA antigen was proportionally and significantly correlated with TAT, EGF and PGE2 in Hp+ patients. The level of TAT was proportionally and significantly correlated with EGF in Hp+ patients but not in Hp– and HP c patients.Conclusions.  These results showed that VacA produced by H. pylori is associated with increased thrombin generation, and that thrombin may play a protective role in H. pylori-associated gastroduodenal disorders.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1523-5378.2005.00323.x
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  • 2
    Electronic Resource
    Electronic Resource
    Expression of E- and P-cadherin during tooth morphogenesis and cytodifferentiation of ameloblasts (1998)
    Springer
    Anatomy and embryology 197 (1998), S. 469-475 
    Details
    ISSN: 1432-0568
    Keywords: Key words Mouse ; Enamel organ ; Enamel knots ; Cell adhesion molecules ; Differentiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Cell-cell adhesion is fundamental in morphogenesis and is known to be mediated by several groups of cell adhesion molecules. Cadherins are a group of such molecules involved in the Ca2+-dependent cell-cell adhesion mechanism and are found in most kinds of tissue. In this study using indirect immunofluorescence microscopy, we analyzed the distribution of two kinds of cadherins, E- and P-cadherin, in developing tooth germs. In the molar tooth germs at the early bud stage, marginal cells of the epithelial tooth bud expressed both E- and P-cadherin, whereas central cells expressed only E-cadherin. At the cap stage, in addition to the cells of the inner and outer enamel epithelium, which outline the enamal organ, cells of the enamel knot, which is thought to control tooth morphogenesis, strongly expressed P-cadherin. The expression of P-cadherin was prominent in the inner enamel epithelium during the early to mid bell stage, and was also evident in the non-dividing cell masses at future cusp tips, which are the so-called secondary enamel knots. In the tooth germ at the late bell stage when the cells of the inner enamel epithelium began to polarize to differentiate into ameloblasts, the polarizing ameloblasts lost P-cadherin and strongly expressed E-cadherin. However, E-cadherin was also lost from polarized ameloblasts at later stages. The stratum intermedium and the stellate reticulum were E-cadherin positive from the bell stage onward even at the stages when the ameloblasts became E-cadherin negative again. These results suggest that the differential expression of E- and P-cadherin during morphogenetic stages plays a role in the regulation of tooth morphogenesis, whereas alteration of E-cadherin expression during later stages of tooth development is related to differentiation and function of the ameloblasts and other cells supporting amelogenesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004290050157
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  • 3
    Electronic Resource
    Electronic Resource
    Osteoconductivity of Porous Titanium Having Young’s Modulus Similar to Bone and Surface Modification by OCP (2007)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 330-332 (Feb. 2007), p. 951-954 
    Details
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: The present study was designed to investigate whether porous titanium (Ti) havingYoung’s modulus similar to bone has osteoconductive characteristics in rat critical-sized calvarialbone defect. The effect of coating by octacalcium phosphate (OCP) was also examined. OCP isknown as a precursor of initial mineral crystals of biological apatite in bones and teeth. Ti powder wasprepared by plasma rotating electrode process in an Ar atmosphere. Then, porous Ti disks, 8 mm indiameter with 1 mm thick, were obtained using the particles ranging from 300 to 500 +m, by sinteringat 1573 K without applied pressure. The disks had about 35 vol% in porosity and about 10 GPa inYoung’s modulus which corresponds to that of human cortical bone. Newly formed bone wasobserved so as to fill the pore up at 12 weeks, confirming the ability to conduct the ingrowths of thebone tissue. Although in vitro study showed that proliferation of mouse bone marrow stromal ST-2cells was inhibited on the dishes coated by OCP rather than the control dish, OCP coating on porousTi seemed to stimulate the bone formation in vivo. Taken together, it seems likely that porous Tihaving Young’s modulus similar to bone shows osteoconductive characteristics to conduct boneingrowths. OCP could be a potential coating agent to assist bone regeneration on porous Ti
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/53/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.330-332.951.pdf
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  • 4
    Electronic Resource
    Electronic Resource
    Apoptosis in mouse taste buds after denervation (1996)
    Springer
    Cell & tissue research 286 (1996), S. 55-62 
    Details
    ISSN: 1432-0878
    Keywords: Key words: Taste bud ; Denervation ; Apoptosis ; Cell death ; TUNEL method ; Glossopharyngeal nerve ; Circumvallate papilla ; Mouse (dd)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Apoptotic cells in the taste buds of mouse circumvallate papillae after the sectioning of bilateral glossopharyngeal nerves were examined by the method of DNA nick-end labeling (TUNEL), together with standard electron microscopy. The taste buds decreased in number and size 3–11 days after denervation and disappeared at 11 days. The TUNEL method revealed only a few positively stained nuclei in normal taste buds but, in those of mice 1–5 days after denervation, the number of positive nuclei had increased to 3–5 times that of taste buds from normal mice. Electron-microscopic observation after denervation demonstrated taste bud cells containing condensed and fragmentary nuclei in a cytoplasm with increased density. The results show that taste bud cells under normal conditions die by apoptosis at the end of their life span, and that gustatory nerve sectioning causes apoptosis of taste bud cells with taste buds decreasing in number and ultimately disappearing.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004410050674
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  • 5
    Electronic Resource
    Electronic Resource
    Olfactory epithelium consisting of supporting cells and horizontal basal cells in the posterior nasal cavity of mice (2000)
    Springer
    Cell & tissue research 299 (2000), S. 313-325 
    Details
    ISSN: 1432-0878
    Keywords: Olfactory epithelium Supporting cells Horizontal basal cells Three-dimensional reconstruction Apoptosis BrdU immunohistochemistry Mouse (DdY)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. The olfactory epithelium of mice generally consists of olfactory cells, progenitors of olfactory cells (globose basal cells), supporting cells, and horizontal basal cells. However, in the dorsal fossa (the roof) of the posterior nasal cavity of mice, we found seven epithelial patches consisting of only non-neuronal cell types, i.e., supporting cells and horizontal basal cells, among the normal olfactory epithelium. The supporting cells occupied three or four layers in the apical to middle regions; in the basal region, horizontal basal cells were localized in a single row adjacent to the basement membrane. Bowman's gland ducts were also present in the epithelium. Neuronal cells (olfactory cells and globose basal cells) were totally absent. The ultrastructure of the supporting cells, horizontal basal cells, and Bowman's glands was essentially similar to that in the normal olfactory epithelium. In the early postnatal period (P1–P7), cell types in the epithelium were the same as those in the normal olfactory epithelium. From P10 to P21, olfactory cells and globose basal cells had disappeared from the olfactory epithelium. At this period, the number of TUNEL-positive cells was significantly higher than that in the surrounding olfactory epithelium; ultrastructurally, many apoptotic figures were observed. This suggests that the epithelium consisting of supporting cells and horizontal basal cells is generated by the apoptotic death of olfactory cells and globose basal cells during postnatal development.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004419900135
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  • 6
    Electronic Resource
    Electronic Resource
    Basal cells in the mouse olfactory epithelium after axotomy: immunohistochemical and electron-microscopic studies (1991)
    Springer
    Cell & tissue research 266 (1991), S. 239-245 
    Details
    ISSN: 1432-0878
    Keywords: Basal cells ; Olfactory epithelium ; Axotomy ; Immunohistochemistry ; Mouse (dd)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The olfactory epithelium of mice after axotomy was investigated to clarify the stem cells of olfactory cells by double immunostaining using antikeratin (MA903) and anti-bromodeoxyuridine (BrdU) antibodies and by conventional electron microscopy. When a single dose of BrdU was given to mice 9 days after axotomy, immunostaining for BrdU was found in the globose basal cells which were negative for MA903, but not in the basal cells proper which were positive for MA903. The BrdU-immunoreactive cells increased 3-to 6-fold over the number of these cells in the controls, indicating active cell proliferation. At other postoperative days (4 and 14 days), fewer BrdU-immunoreactive cells were found. Furthermore, three pulses of BrdU resulted in numerous BrdU-immunolabelings in the globose basal cells and a few in the basal cells proper. There was no detectable difference in the number of labeled basal cells proper in operated and unoperated mice. In the electron micrographs 9 days after axotomy, the basal cells proper, flat-shaped in unoperated mice, appeared cylindrical or pyramidal in shape and the globose basal cells often lay between the basal cells proper. In unoperated controls, the globose basal cells were located above the flat-shaped basal cells proper. The results suggest that the stem cells of the olfactory cells are globose basal cells and not basal cells proper, and that the shape of basal cells proper changes in relation to the active proliferation of stem cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00318179
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  • 7
    Electronic Resource
    Electronic Resource
    Induction of apoptosis by colchicine in taste bud and epithelial cells of the mouse circumvallate papillae (2000)
    Springer
    Cell & tissue research 302 (2000), S. 391-395 
    Details
    ISSN: 1432-0878
    Keywords: Taste bud Colchicine Apoptosis Epithelial cell Circumvallate papilla Microtubule Mouse (dd)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Apoptotic cells in the taste buds and epithelia of mouse circumvallate papillae after colchicine treatment were examined by the methods of in situ DNA nick-end labeling, immunocytochemistry, and electron microscopy. After colchicine treatment, numerous positive cells appeared in the taste buds by DNA nick-end labeling, and some epithelial cells in the basal and suprabasal layers in and around the circumvallate papillae also revealed positive staining. Condensed and fragmented nuclei with a high density were occasionally found in the taste bud cells and in the basal and suprabasal layer epithelial cells by electron-microscopic observation. An immunocytochemical reaction for tubulin revealed weak staining in taste bud cells, because of the depolymerization of microtubules, and a decrease of the microtubules in the taste bud cells was observed by electron microscopy. These results indicate that colchicine treatment of mice induces the apoptosis of taste bud and epithelial cells in the circumvallate papillae and dorsal epithelial cells around the circumvallate papillae.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004410000294
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