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Analysis of RNA and protein synthesis in temperature-sensitive mutants of Tetrahymena pyriformis (1978)

Hallberg, Richard L. ; Gritz, Linda R. ; Sylvan, Paul

Springer

Molecular genetics and genomics 158 (1978), S. 305-312

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ISSN: 1617-4623

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Summary Temperature-sensitive mutants of Tetrahymena pyriformis which had previously been selected for their inability to grow at 38°C but which grew normally (or near normally) at 30°C were characterized with respect to their patterns of RNA and protein accumulation at both the permissive and nonpermissive temperatures. Out of 116 such mutants, the majority (72) acted like wild type for these accumulations during a 3 h labelling period although some of them stopped dividing during this time. The remainder exhibited a variety of altered phenotypes for the rate, extent, and timing of RNA and/or protein accumulation. Those mutants which exhibited selective inhibition of RNA accumulation, and were thus potential ribosomal RNA (rRNA) mutants, were further characterized by examining patterns of protein and RNA synthesis in cells starved at the permissive temperature, but re-fed at the permissive and non-permissive temperatures. At least five different types of mutants as defined by patterns of protein and RNA synthesis in refed cells were identified. Direct analysis of the RNA synthesized in cells from 2 of these types of mutants showed that in 5 out of 6 cases rRNA synthesis and/or processing was inhibited within 30 min after shifting to the non-permissive temperature. The other mutant examined was found to show a delayed inhibition of rRNA synthesis.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00267202

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Ribosome biosynthesis in Tetrahymena thermophila. IV. Regulation of ribosomal RNA synthesis in growing and growth arrested cells (1979)

Sutton, Claudia A. ; Sylvan, Paul ; Hallberg, Richard L.

New York, NY [u.a.] : Wiley-Blackwell

Journal of Cellular Physiology 101 (1979), S. 503-513

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ISSN: 0021-9541

Keywords: Life and Medical Sciences ; Cell & Developmental Biology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Medicine

Notes: Three parameters involved in the production of new ribosomal RNA (rRNA) were measured in Tetrahymena thermophilia: (i) the rate of synthesis of the rRNA precursor, (ii) the rate of processing of the RNA precursor and rRNA intermediates and (iii) the efficiency of utilization of the rRNA precursor in producing mature ribosomal RNA. These parameters were measured in cells in exponential growth and in cells starved in a dilute salt solution. Growing cells synthesize rRNA 20 times faster and process rRNA precursors and intermediates 10 to 15 times more rapidly than do starved cells. Both utilize their rRNA precursors with an efficiency of one in converting them to mature rRNA.

Additional Material: 8 Ill.