ISSN:
1432-1203
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Description / Table of Contents:
Zusammenfassung Menschliche Lymphocyten, die am 6., 9. und 12. Tag nach Pockenschutzimpfung kultiviert wurden, zeigten keine sicher verwertbaren Chromosomenanomalien. Nach Infektion menschlicher Lymphocytenkulturen hemmt Vaccinevirus die Mitosen und zerstört die Zellen in toto parallel zur Dauer der Einwirkung. Numerische und strukturelle Veränderungen an den Chromosomen wurden nicht beobachtet.
Notes:
Summary Contradictory views are expressed in the literature on the question as to whether viruses induce anomalies in chromosomes. Vaccinia viruses in particular are generally stated to cause chromatid and chromosome breakage in L-cells (human embryonal lung tissue). The subject of the present paper is an experimental investigation of the effect of vaccinia viruses on the chromosomes of human blood lymphocytes. In the in vivo portion of this investigation the chromosomes were examined during and after viremia subsequent to vaccination. Only two of the eight vaccinated children examined showed a larger number of chromosome breaks during viremia than unvaccinated controls. This might possibly be due to different degrees of viremia, although local and general reactions were the same. Other causes might also be sought in vivo or in vitro, such as preparation of the chromosomes, or an unnoted mycoplasma infection of the lymphocyte cultures. In the in vitro portion of this investigation, cultures of human lymphocytes were infected with vaccinia viruses at different stages of incubation. In culture the virus concentrations exhibited a rapid initial decrease, which gradually tapered off during the period of observation. No increases in virus concentration were noted. The longer the period of infection with caccinia virus, the less growth shown by the lymphocytes, and the more mitotic cells with in toto destruction of the chromosome. Anomalies in individual chromosomes were not observed any more frequently than in controls.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00287154
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