ISSN:
1745-4522
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Silver ion-high performance liquid chromatography (Ag-HPLC) and capillary gas chromatography (GC) were combined to quantify total trans-octadecenoic acid (18:1t) in diet composites and oils. The 18:1 cis and trans fatty acid methyl esters (FAME) of saponified and methylated (BF3/methanol) oils and food composite total lipid extracts were separated by Ag-HPLC (ChromSpher5 Lipids column); total 18:1t in the trans fraction was quantified by GC (105m × 0.25mm SP-2560 capillary column). Positional isomers 18:1c and 18:1t which coeluted in direct GC were clearly separated by preparatory Ag-HPLC. Quantitative recovery of 18:1t from a standard mixture of FAME, an oil, and a diet composite was obtained for additions of 4–57 mg. The analytical range for total 18:1t was 1.6–260 mg/mL per aliquot of total lipid extract or oil. Relative standard deviations were 〈 6% for food composites and 〈 3% for oils and pure standards.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1745-4522.1997.tb00091.x
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