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  • 1
    Electronic Resource
    Electronic Resource
    T-Cell Response to Purified Protein Derivative after Removal of Langerhans' Cells from Epidermal Cell Suspensions Containing Keratinocytes Expressing Class II Transplantation Antigens (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 28 (1988), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: In a previous study we observed that human epidermal cell (EC) suspensions containing HLA-DR-expressing keratinocytes showed an amplified T-cell response to purified protein derivative (PPD). To evaluate further the possible immunological importance of class II transplantation antigens on keratinocytes we have compared the T-cell response to PPD in the presence of the following stimulator cells: EC suspensions from normal skin, or EC from tuberculin-reactive skin with or without removal of Langerhans' cells. The proliferation of purified T lymphocytes from peripheral blood in response to PPD in the presence of various concentrations of autologous EC was measured by [3H]thymidine incorporation on day 6. In 3 experiments out of 4 the EC from tuberculin-reactive skin, containing 28–76% HLA-DR-expressing cells as judged by immunocytochemistry (which also revealed fairly numerous HLA-DQ/ -DP-expressing keratinocytes and a slight increase in CD36- and CD4- but not CD1-expressing cells), induced a more pronounced T-cell response to PPD than did normal EC. This was not the case in the fourth experiment, in which a small number of HLA-DR- (15%) and few if any HLA-DQ-/-DP-expressing keratinocytes were found. Immunomagnetic removal of CD1-reactive Langerhans' cells from the tuberculin-reactive EC suspensions resulted in a reduction of the T-cell response to PPD, in most cases down to background level (T cells alone + PPD). This study does not support the hypothesis that HLA-DR-expressing keratinocytes can in themselves act as antigen-presenting cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1988.tb01500.x
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  • 2
    Electronic Resource
    Electronic Resource
    Class II Transplantation Antigens in the Late Human Skin Tuberculin Reaction (1985)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 22 (1985), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The presence of class II transplantation antigens on keratinocytes and the phenotypes of the inflammatory cells in the late human skin tuberculin reaction were analysed with immunohistochemical double staining techniques in frozen sections of skin biopsies taken 10-45 days after intradermal purified protein derivative (PPD) injection. Dermal cell infiltrates decreased with time but were found throughout the observation period. Most of the cells in the perivascular infiltrates expressed HLA-DR antigens. Some of these cells are probably ‘activated’ macrophages, since they expressed OKM1 and OKT9 antigens in consecutive sections. Another less frequent cell population which reacted with RFD1 antibodies are presumably interdigitating cells. About half the perivascular cells were anti-Leu 3a-positive (T ‘helper/inducer’ phenotype). HLA-DR but not HLA-DO antigens were detected on keratinocytes. This acquired expression of HLA-DR antigens on the epithelial cells disappeared between 17 and 30 days. If HLA-DR-expressing keratinocytes have immunoregulatory functions these might be different from those of other HLA-DR-expressing cell types that also express HLA-DO molecules.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1985.tb01875.x
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  • 3
    Electronic Resource
    Electronic Resource
    Amplification of T-Cell Response to PPD by Epidermal Cell Suspensions Containing HLA-DR-Expressing Keratinocytes (1987)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 26 (1987), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The biological importance of the presence of class II transplantation antigens on highly differentiated epithelial cells such as keratinocytes in certain conditions, is still unknown. We have therefore investigated the antigen-presenting capacity of separated human epidermal cells obtained from tuberculin-reactive skin 6 days after intradermal injection of purified protein derivative (PPD). Earlier studies have shown a high percentage of HLA-DR-expressing keratinocyles at this time. Peripheral adherent blood cells were used as control stimulator cells a d highly purified peripheral blood T lymphocytes as responder cells. The T-cell proliferation in response to PPD in the presence of autologous epidermal cells from normal and tuberculinreactive skin was measured by [3H]thymidine incorporation on day 6. The latter cell population, 76-86% of which consisted of HLA-DR-expressing cells as judged by immunocytochemistry, induced a greater T-cell response to PPD than do normal epidermal cells. This discrepancy in the T-cell proliferation could not be explained by a difference in ihe numbers of anti-Leu 6 or anti-HLA-DQ-reactive Langerhans cells. The present data indicate that epidermal cell suspensions containing HLA-DR-expressing keratinocytes induce a greater T-cell response to PPD than do normal epidermal cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1987.tb02227.x
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  • 4
    Electronic Resource
    Electronic Resource
    Class II Transplantation Antigens: Distribution in Tissues and Involvement in Disease (1985)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 21 (1985), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1985.tb01823.x
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  • 5
    Electronic Resource
    Electronic Resource
    Human Keratinocytes Express HLA-DR Antigens in the Tuberculin Reaction (1984)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 19 (1984), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The cellular response in the human skin tuberculin reaction was studied with immunohistochemical double-staining techniques in frozen sections of skin biopsies taken 6 h to 8 days after intradermal PPD injections. Cell infiltrates were observed from day 2 onwards and increased in size up to 4 days. Most of the infiltrating cells reacted with anti-Leu 3a (T ‘helper/inducer’ phenotype) antibodies. In contrast to normal epidermis, not only Langerhans cells but also keratinocytes expressed MLA-DR antigens from day 4 onwards. The induction of HLA-DR antigens on keratinocytes may be secondary to T-cell activation. Since the HLA-DR expression on keratinocytes appeared late in the tuberculin reaction, the function may be to suppress rather than enhance the immune response.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1984.tb00910.x
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  • 6
    Electronic Resource
    Electronic Resource
    Appearance of Anti-HLA-DR-Reactive Cells in Normal and Rheumatoid Synovial Tissue (1981)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 14 (1981), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The reactivity of rabbit anti-HLA-DR antigen antibodies with cells in normal and rheumatoid synovial tissue was investigated by indirect immunofluorescence on frozen sections of tissue. The antibodies reacted with a significant proportion of the synovial lining cells of both normal and rheumatoid synovial tissue, with endothelial cells, and with a number of, most probably, migratory cells. After dispersion of cells from rheumatoid synovial tissue by digestion with collagenase and DNase, adherent cells of both a macrophage-like and a dendritic appearance reacted with the anti-HLA-DR antigen antibodies. The adherent cells were also found to be potent stimulators in the allogeneic MLR. In addition, it was found that a high percentage of T lymphocytes from both peripheral blood and synovial tissue of rheumatoid patients bound anti-HLA-DR antibodies. The present data suggest a role for synovial lining cells in HLA-D-locus-dependent events of importance in the pathogenesis of rheumatoid arthritis and other joint diseases and point to the need for further investigations on T lymphocytes derived from the site of inflammation in the study of rheumatoid arthritis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1981.tb00198.x
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  • 7
    Electronic Resource
    Electronic Resource
    Expression of HLA-DQ Antigens on Keratinocytes in Borrelia Spirochete-Induced Skin Lesions (1986)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 23 (1986), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Skin biopsies were investigated with two different immunohistochemical techniques. thus revealing HLA-DO antigens on HLA-DR-expressing keratinocytes in the late skin manifestations of a Borrelia spirochete infection. In the early skin lesions only HLA-DR antigens were present on the keratinocytes.’The invariant γ chain of class II transplantation antigens was observed on keratinocytes in 1:5 of the late cases. Upon penicillin treatment detectable HLA-DR and HLA-DQ antigens disappeared completely From the keratinocytes. Furthermore, the mononuclear cell infiltrates dominated by anti-Leu 1 and anti-Leu 3a-reactive cells and containing many cells with markers for activation (HLA-DR. HLA-DO. transferrin. and interleukin 2 receptors) diminished markedly. The possibility that the expression of different class II transplantation antigens on keratinocytes might reflect separate functional demands of these cells or an altered immunological reactivity in the host, is discussed. the precise functional role of the temporary expression of the class II antigens on non-lymphoid cells, however, remains an enigma.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1986.tb01979.x
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  • 8
    Electronic Resource
    Electronic Resource
    Effect of UV-irradiation on immunological and histochemical markers of langerhans cells in normal appearing skin of psoriatic patients (1981)
    Springer
    Archives of dermatological research 272 (1981), S. 171-176 
    Details
    ISSN: 1432-069X
    Keywords: UV-irradiation ; Psoralens ; Langerhans cells ; ATPase ; Ia-antigens ; OKT-o-antigens
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00510411
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