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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 665 (1992), S. 0 
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of rapid methods and automation in microbiology 3 (1995), S. 0 
    ISSN: 1745-4581
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Membrane fractions obtained from Escherichia coli, Gluconobacter oxydans, and Acetobacter xylinum significantly stimulated rapid growth of Listeria monocytogenes (strains 101, 103, and Scott A) under atmospheric conditions of the OmniSpec Bioactivity System. L. monocytogenes demonstrated a shorter lag phase and faster growth compared to culture systems without the membrane fractions as determined by shorter color detection times, the durations of time for a significant color change to occur. The growth stimulating effect increased as the concentrations of membrane fractions increased. The use of membrane fractions to recover facultative Listeria spp. also lowered the detection limit of the method to 〈 10 cells/ml by increasing small cell numbers to the detectable level (107 cells/m) faster under aerobic conditions. The application of membrane fractions with the OmniSpec Bioactivity System shortened the detection time for the bacteria by 0.5–10 h, depending on the specific membrane and initial cell concentrations, compared to the conventional OmniSpec method. This method is very useful as a tool for studying food microbiology. Membrane fractions produced in our laboratory effectively dissipated oxygen in Fraser broth and had growth-enhancing activities comparable to that of commercial OxyraseTM.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of rapid methods and automation in microbiology 3 (1994), S. 0 
    ISSN: 1745-4581
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Bacterial membrane fraction biocatalysts obtained from Escherichia coli (E-8), Gluconobacter oxydans (Gox) and Acetobacter xylinum (Acx), as well as the commercial oxygen scavenger OxyraseTM, at concentrations of 0.1–2.0 U/ml effectively stimulated cell growth of Lactobacillus bulgaricus and Sreptococcus thermophilus, and lactic acid production during yogurt fermentation. The membrane fractions scavenged oxygen in the fermented milk to an optimal oxygen tension for growth of yogurt cultures. The yogurt culture populations with membrane fraction(s) increased faster than those without. Total counts in the presence of yogurts with Oxyrase, E-8, Gox, and Acx were 0.5–1, 0.5, 1.5, or 1.2 log cycles, respectively, greater than counts of the control after 3 h of fermentation. The commercial membrane fraction Oxyrase reduced the fermentation time by 1 h needed to reach pH 4.5 compared to the controls, while E-8, Gox, and Acx reduced time by about 0.5 h, 1.5 h and 1–1.5 h, respectively, depending on the membrane concentrations. The titratable acidity was corresponded well with the reduction in pH. Ratios of rods to cocci among the samples with and without membrane fraction supplementation were not different (P 〉 .05). Each membrane fraction biocatalyst enhanced the depletion rate of dissolved oxygen in a yogurt mix differently.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of rapid methods and automation in microbiology 3 (1994), S. 0 
    ISSN: 1745-4581
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A commercial oxygen reducing membrane fraction obtained from Escherichia coli (OxyraseTM) has been shown in our laboratory to stimulate growth of many facultative anaerobic organisms, including starter cultures. The purpose of this study was to isolate “Food Grade” membrane fractions from Acetobacter xylinum and Gluconobacter oxydans and to compare their activities with the membrane fractions from E. coli prepared in our laboratory and Oxyrase. Cells were grown in afermentor, harvested by centrifugation, and disrupted by a French press (ca. 15,000 psi). The suspension was fractionated and filtered before use. The maximum activity of membrane fractions of Gluconobacter and Acetobacter were from cultures grown to 24 and 44 h, respectively. Formate, lactate, succinate, and α-glycerophosphate were tested as H-donors for enzyme activity. When lactate and α-glycerophosphate were used as substrates, Acetobacter membrane fractions were able to deplete 100% oxygen in 5 min. It required 8 and 30 min, respectively, when succinate and formate were used. Gluconobacter membrane fractions were effective with lactate and α-glycerophosphate. With succinate and formate, they took about 60 min or longer to reduce O2 completely. E. coli membrane fractions made in our laboratory depleted O2 more effectively (1 min) than Oxyrase (3–5 min) when formate was used as a H-donor.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of rapid methods and automation in microbiology 9 (2001), S. 0 
    ISSN: 1745-4581
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Efficacy of the bacterial membrane fractions to hasten the buttermilk fermentation was evaluated. Membrane fraction biocatalysts, at 0.1–2.0 U/mL, obtained from Escherichia coli (E-8), Gluconobacter oxydans (Gox), and Acetobacter xylinum (Acx) and a “OxyraseTM” effectively stimulated cell growth of buttermilk cultures (Lactococcus lactis, L. cremoris, and Leuconostoc spp.) and lactic acid production of the buttermilk fermentation. The membrane fractions scavenged oxygen in the fermenting milk to provide an optimal oxygen tension for growth of the cultures. OxyraseTM and E-8 membrane fractions significantly increased the rate of pH reduction by stimulating lactic acid production to reach the desirable conditions (pH 4.8 and 0.85% acidity as lactic acid) by 0.4–3.5h and 0.9–3.3h, respectively, depending on the concentrations, which was faster than the controls. Gox and Acx, at 0.1–1.0 U/mL stimulated the production to finish 1.5 to 2.5h faster than that of the control. Each membrane fraction biocatalyst depleted dissolved oxygen in the buttermilk mix differently. Total counts of the cultures with OxyraseTM and E-8 membrane fractions at 0.5 U/mL were significantly higher than those of the control by 0.5–1.0 log cycle during hours 3–7.
    Type of Medium: Electronic Resource
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