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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Queueing systems 36 (2000), S. 107-123 
    ISSN: 1572-9443
    Keywords: synchronization queue ; output process ; Markov renewal process ; matrix analytical method ; phase-type distribution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science
    Notes: Abstract In this paper, we consider a synchronization queue (or synchronization node) consisting of two buffers with finite capacities. One stream of tokens arriving at the system forms a Poisson process and the other forms a PH-renewal process. The tokens are held in the buffers until one is available from each flow, and then a group-token is instantaneously released as a synchronized departure. We show that the output stream of a synchronization queue is a Markov renewal process, and that the time between consecutive departures has a phase type distribution. Thus, we obtain the throughput of this synchronization queue and the loss probabilities of each type of tokens. Moreover, we consider an extended synchronization model with two Poisson streams where a departing group-token consists of several tokens in each buffer.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Journal of plant research 109 (1996), S. 331-334 
    ISSN: 1618-0860
    Keywords: Arabidopsis thaliana ; Hygromycin phosphotransferase gene (hpt) ; Particle bombardment ; Transgenic plant
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Root explants ofArabidopsis thaliana ecotype C24 were bombarded with the plasmid pCH harboring the hygromycin phosphotransferase gene (hpt). A selection condition with post-bombardment culture of 3 days followed by culture with 20 mgl−1 hygromycin gave the highest yield of transformants. More than 44% of explant clumps formed transformant shoots.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1573-9368
    Keywords: cultured liverwort cells ; hygromycin phosphotransferase (HPT) ; Marchantia polymorpha ; particle bombardment ; stable transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Suspension-cultured cells (A-18 line) of the liverwortMarchanta polymorpha were bombarded by a pneumatic particle gun with plasmid pCH harbouring the hygromycin phosphotransferase (HPT) gene (hpt) under the control of the cauliflower mosaic virus (CaMV) 35 S promoter and the nopaline synthase polyadenylation region. Nine weeks after bombardments, 128 hygromycin-resistant calluses were obtained from an approximate total of 7×106 cells. Ten cell lines chosen randomly were analysed further. Southern blot analysis showed that all of the ten lines contain thehpt gene in the genome, demonstrating that these lines are transformants. An HPT enzyme activity assay confirmed the expression of the gene in all of the transformant lines.
    Type of Medium: Electronic Resource
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