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  • 1
    ISSN: 1520-4812
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1574-695X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Abstract Polyclonal rabbit anti-idiotypic antibody (anti-Id) against the protective monoclonal antibody specific to the flagella of Clostridium chauvoei was produced, purified, and characterized. Anti-Id inhibited the binding of its related monoclonal antibody to the flagellar antigen, suggesting that the anti-Id bore an internal image of the flagellar antigen. When mice were immunized with anti-Id intraperitoneally, the survival rate increased significantly, compared with mice immunized with normal rabbit IgG (P 〈 0.01), and specific anti-flagellar antibodies were induced.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-2323
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. To determine the indications for limited colon cancer surgery in each location, we reviewed the arterial branching patterns and lymph node metastases along the course of specific vascular trunks in 344 colon cancer patients who had undergone preoperative angiography and colectomy with lymph node dissection. Our conclusions are follows: Because the ileocecal artery always arises from the superior mesenteric artery and lymph node metastases of cecum cancer were limited to nodes along the ileocolic artery, cecum cancer can be cured by ileocecal resection. The right colic artery has various origins, and ascending colon cancer shows various patterns of lymph node metastases. Therefore a right hemicolectomy should be performed for ascending colon cancer. The middle colic artery forks into right and left branches, and each branch has different branching variations. If the right colic and middle colic arteries have a common trunk, a right hemicolectomy should be performed for transverse colon cancer on the right side. If the left branch of the middle colic artery has an independent replaced origin, lymph node dissection should be modified according to the variant origin. If the left colic artery and the first sigmoidal artery have a common trunk, the lymph nodes along the common trunk should be removed for sigmoid colon cancer and for descending colon cancer. Of the patients with sigmoid colon cancer, 6.3% also had lymph node metastases along the superior rectal artery. Given that the lymph nodes along the superior rectal artery are skeletonized, sigmoid colon cancer can be also cured by partial sigmoidectomy.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Journal of cancer research and clinical oncology 106 (1983), S. 21-26 
    ISSN: 1432-1335
    Keywords: Mouse colon adenocarcinoma 38 ; Indomethacin ; Prostaglandin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The anti-inflammatory drug indomethacin was tested for antitumor activity against transplantable mouse colon adenocarcinoma 38 (colon 38). Groups of BDF1 mice (C57BL/6xDBA/2) were given intraperitoneal injections of this drug beginning on the 6th day after subcutaneous implantation of the tumor and continued for 4 to 8 days. In other groups of mice, identical treatment was delayed until the 16th day after implation of the tumor. The higher antitumor activity against colon 38 was obtained with earlier initiation of treatment, indicated by decreased growth of the tumor and increased life span of the host. The later initiation of the treatment produced less antitumor activity. The antitumor activity was, however, less than that of 5-fluorouracil, which was used as a positive control drug. The two drugs in combination produced few advantages over 5-fluorouracil alone using the dose schedule designed in the present experiment. Indomethacin treatment significantly reduced prostaglandin E and F levels in the tumor tissue, but 5-fluorouracil did not. It seems likely that the inhibition of prostaglandin biosynthesis by indomethacin underlies the antitumor effect of this drug on colon 38.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-2323
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé Lors de la chirurgie gastrique pour cancer, il arrive que l'artère hépatique gauche, lorsqu'elle prend naissance de l'artère coronaire stomachique (AHG/CS), soit dévascularisée lors de la ligature de l'artère coronaire stomachique ou qu'elle soit sectionnée lors de la dissection. La dévascularisation peut parfois Être à l'origine de troubles hépatiques postopératoires. Chez 141 patients ayant eu une étude artériographique avant d'avoir une gastrectomie pour cancer, 28 patients avaient une AHG/ CS. Parmi ceux-ci, l'AHG/CS a été sectionnée dans 15 cas. La fonction hépatique postopératoire a été évaluée par les dosages de la lactate déshydrogénase, des transaminases glumatopyruviques et glutamo-oxalo-acétiques. Les modifications les plus importantes ont été enrégistrées chez les patients qui avaient eu une ligature de leur AHG/CS, alors que ces mÊmes fonctions hépatiques n'étaient pas perturbées chez les patients ayant une AHG/CS non sectionnée ou n'ayant pas une AHG/CS. En plus, on a remarqué que les perturbations de la fonction hépatique étaient proportionnelles au territoire irrigué par l'AHG/CS. Ces perturbations, cependant, étaient habituellement transitoires et, le plus souvent, ces patients ont récupéré une fonction hépatique normale en moins de 7 jours.
    Abstract: Resumen La lesión o división de la arteria hepática izquierda que se origina en la arteria gástrica izquierda (AHAG) ocurre ocasionalmente durante una operación por cáncer gástrico, lo cual puede causar disfunción hepática postoperatoria. Se revisaron 141 pacientes sometidos a gastrectomía por cáncer gástrico después de angiografía preoparatoria. De los 28 pacientes con AHAG, en 15 se dividió la AHAG y no en los restantes. La función hepática postoperatoria estimada mediante la determinación de los cambios en los niveles de deshidrogenasa láctica (LDH), transaminasa glutámica oxaloacética y transaminasa glutámico pirÚvica (GPT). La mayor alteración en la función hepática fue observada en los pacientes en quienes se dividió la AHAG, en tanto que los cambios en la función hepática con preservación de la AHAG fueron similares a los observados en los pacientes sin AHAG. Fue notorio que entre mayor el área irrigada por la AHAG, mayor el grado de alteración en la función hepática. La disfunción hepática tuvo carácter transitorio, y la función hepática se normalizó en los primeros siete días postoperatorios.
    Notes: Abstract An aberrant left hepatic artery, arising from the left gastric artery (LHLG), either as an accessory or replacing the left hepatic artery, is occasionally seen in patients with gastric cancer. Resection of LHLG, as part of the gastrectomy procedure, may cause liver dysfunction. The surgical records of a group of 141 patients who had undergone gastrectomy for gastric cancer following preoperative angiography were reviewed. Twenty-eight of the patients had an LHLG preoperatively; in 15 the artery was severed during surgery and in the remainder it was preserved. Postoperative liver function was assessed by measuring changes in lactate dehydrogenase, glutamic oxaloacetic transaminase, and glutamic pyruvic transaminase activities. The greatest changes in liver function were observed in patients in whom the LHLG had been severed; the changes in liver function among patients whose LHLGs were preserved were similar to those in patients who had no LHLG. Notably, the wider the area fed by the LHLG, the greater was the change in liver function. The observed liver dysfunctions were transient, however, and normal function resumed within 7 days after operation.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1432-2323
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé Dans cette étude, nous avons essayé de déterminer s'il y avait un rapport entre le type d'anastomose et l'incidence de sténose anastomotique après la chirurgie du tube digestif supérieur. Nous rapportons aussi notre expérience de dilatation par ballonnet. Nous avons déterminé l'incidence des sténoses anastomotique chez les patients suivis dans notre service depuis 17 ans, ayant eu soit une anastomose oesogastrique après oesophagectomie subtotale pour cancer de l'oesophage, soit une oesojéjunostomie après une gastrectomie proximale ou totale pour cancer gastrique. Parmi les 283 cas d'oesojéjunostomies, il y a eu sept sténoses (après avoir éliminé trois cas de récidive). Leur fréquence après suture conventionnelle était de 1.8% et après anastomose mécanique de 4.6%. Il y avait 17 sténoses parmi les 56 anastomoses oesogastriques. La fréquence après suture conventionnelle était de 28.6% et après suture mécanique de 50.0%. Le diamètre de l'anastomose oesojéjunale a été mésuré par un transit baryté un mois ou plus après l'opération. Le diamètre moyen après agraphage mécanique était de 11.9 ± 2.9 mm, alors que le dimètre moyen après anastomose oesogastrique en un plan sous-muqueux (selon Jourdan) était de 19.8 ± 2.2 mm et après les points en “U” de 19.0 ± 2.0 mm. La sténose a été dilatée dans 29 cas (19 cas de cancer oesophagien et 10 cas de cancer gastrique. Après des séances répetées, nous avons pu dilater avec succès toutes les sténoses bénignes sans aucune complication. Nous croyons que la dilatation est une méthode facile, sûre et efficace dans la thérapeutique des sténoses anastomotiques du tube digestif supérieur.
    Abstract: Resumen Informamos nuestro estudio sobre la correlación entre el tipo de anastomosis y la incidencia de estenosis anastomótica en el tracto gastrointestinal; también informamos nuestra experiencia con la dilatación por medio de balón. Hemos examinado la incidencia de estenosis en pacientes sometidos a esofagectomía subtotal con anastomosis entre el esófago y el estómago por cáncer esofágico y a gastrectomía proximal o total con esófagoyeyunostomía por cáncer gástrico en los últimos 17 años. En el grupo de 283 casos de esofagoyeyunostomía se observaron 7 casos de estenosis (excluyendo 3 casos de recurrencia del cáncer): anastomosis convencional 1.8%; anastomosis con grapas (“staples”) 4.6%. Hubo 17 casos de estenosis entre 56 que tuvieron anastomosis entre el esófago y el estómago después de esofagectomía: anastomosis convencial 28.6%; anastomosis con grapas 50.0%. Se hizo la estimación del diámetro de la esófago-yeyunostomía mediante un estudio con bario al mes o más después de efectuada la operación. El diámetro promedio de la anastomosis cuando se usó el suturador mecánico fue 11.9 ± 2.9 mm, et tanto que el diámetro promedio de las anastomosis manuales con el método de Jourdan de una sola capa serosubmucosa fue 19.8 ± 2.2 mm y de 19.0 ± 2.0 mm con la anastomosis con suturas verticales de colchonero. Se utilizó la dilatación con balón en 29 casos de estenosis anastomótica, 19 casos de cáncer esofágico y 10 de cáncer gástrico. Mediante dilataciones repetidas logramos resultados eficaces con las estenosis benignas y no se presentaron complicaciones graves. Creemos que la dilatación por medio de balón constituye una modalidad terapéutica sencilla, segura y eficaz para el manejo de las estenosis anastomóticas del tracto gastrointestinal superior.
    Notes: Abstract We report our study on the correlation between the types of anastomosis and the incidence of anastomotic stricture formation in the upper gastro-intestinal tract. Our experience with balloon dilatation is also reported. We examined the incidence of stricture formation among patients who had an anastomosis between the esophagus and stomach following subtotal esophagectomy for esophageal cancer, and esophagojejunostomy following proximal or total gastrectomy for gastric cancer in the past 17 years. Among 283 patients undergoing esophagojejunostomy, 7 cases of stricture (excluding 3 cases of cancer recurrence) were observed (conventional anastomosis 1.8%; stapling anastomosis 4.6%). There were 17 cases of stricture among 56 patients who had anastomosis between the esophagus and stomach following subtotal esophagectomy (conventional anastomosis 28.6%; stapling anastomosis 50.0%). One month or more after the operation, the diameter of the esophagojejunostomy was estimated using a barium study. The mean diameter of the anastomosis using the stapling method was 11.9 ± 2.9 mm, whereas the mean diameter of serosubmucosal single layer hand-sewn anastomosis (Jourdan's) was 19.8 ± 2.2 mm, and that of vertical mattress hand-sewn anastomosis was 19.0 ± 2.0 mm. Balloon dilatation was used in 29 patients with anastomotic stricture of the upper gastro-intestinal tract (esophageal cancer, 19 patients, gastric cancer, 10 patients). With repeated dilatation, we were able to obtain satisfactory efficacy for benign strictures and there were no severe complications. We believe that balloon dilatation is an easy, safe and effective therapy for anastomotic stricture of the upper gastro-intestinal tract.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1432-2323
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé Les émulsions de graisses étant principalement absorbées par les voies lymphatiques, nous avons étudié, expérimentalement et en clinique, l'efficacité d'une émulsion de 5-fluorouracil (5-FU), administrée per os, comme traitement adjuvant des cancers gastriques, surtout en cas de métastases ganglionnaires. L'étude chez le rat a montré que la concentration de 5-FU dans les ganglions régionaux est plus élevée et persiste plus longtemps après administration orale d'une émulsion de 5-FU qu'après administration d'une solution aqueuse. Chez 13 malades atteints de cancer gastrique et qui avaient reçu per os, 2 heures avant l'opération, soit une émulsion, soit une solution de 5-FU, la concentration du produit dans les ganglions régionaux est plus élevée après ingestion de l'émulsion. Au cours des 3 dernières années, 123 malades atteints de cancer gastrique ont reçu une émulsion de 5-FU pendant 1–3 semaines avant l'opération. L'examen histologique des ganglions a montré d'importantes lésions de nécrose et dégénérescence dans 62% des métastases ganglionnaires. Ces résultats suggèrent que l'administration orale préopératoire d'une émulsion de 5-FU peut être une thérapeutique adjuvante efficace dans la chirurgie des cancers gastriques, surtout chez les malades présentant des métastases lymphatiques.
    Notes: Abstract Based on the propensity of fat emulsion to be absorbed mainly into lymphatic capillaries and regional lymph nodes, we studied experimentally and clinically the effectiveness of oral administration of a 5-fluorouracil (5-FU) emulsion as an adjuvant to surgery in the treatment of gastric cancer, especially when there were lymph node metastases. Experiments in rats showed that drug concentration in the regional lymph nodes was higher and remained longer after oral administration of 5-FU emulsion than after oral administration of an aqueous solution of 5-FU. Furthermore, measurement of the concentration of 5-FU in the regional lymph nodes of 13 patients with gastric cancer who had been given the 5-FU emulsion or 5-FU in water orally 2 hours before surgery showed that the mean 5-FU level was higher in the patients that received the emulsion than in those who received the aqueous solution. In the past 3 years, we have administered 5-FU emulsion to 123 patients with gastric cancer for a period of 1–3 weeks before surgery, and examined histologically the effect of this therapy on the met astatic foci in the lymph nodes. A positive change, such as marked necrosis or marked degeneration, was found in 62% of the metastatic lesions. The results suggest that preoperative oral administration of 5-FU emulsion may be effective as an adjuvant to surgery for gastric cancer, particularly in patients with metastases to lymph nodes.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1432-0843
    Keywords: Key words Etoposide ; Toxicity ; Intraperitoneal administration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  We compared the safety and efficacy in mice with peritoneal carcinomatosis of two etoposide formulations: an aqueous solution (Etp-sol) and particles suspended in oil (the addition products of iodine and the ethyl esters of the fatty acids obtained from poppy-seed oil (Lipiodol) or sesame oil; Etp-oil). We also investigated tissue distribution of etoposide in rats treated with Etp-oil and Etp-sol. Etoposide was injected intraperitoneally at concentrations ranging from 52 to 392 mg/kg (increasing geometrically by a factor of 1.4). The 50% lethal dose (LD50), determined over a 2-week period of observation, was 135 mg/kg for Etp-oil and 108 mg/kg for Etp-sol. Autopsy findings included macroscopic intestinal bleeding, necrosis of the intestinal mucosa, and pulmonary congestion in mice from both treatment groups. In the efficacy trials. 106 P388 leukemia cells were transplanted into CDF1 male mice, and Etp-oil and Etp-sol were injected at doses of 20 mg/kg and 80 mg/kg. In the groups receiving the 20 mg/kg dose, 11 of 19 mice in the Etp-oil group survived to day 60 compared with 3 of 20 mice in the Etp-sol group. Toxicity-related deaths occurred in 1 of 20 mice treated with 80 mg/kg Etp-oil and in 8 of 20 mice treated with 80 mg/kg Etp-sol. No cancer-related deaths were associated with the 80 mg/kg dose in either treatment group. Our findings showed that the Etp-oil was associated with a lower toxicity and a higher efficacy than the Etp-sol. To evaluate tissue distribution, rats were injected intraperitoneally with 5 mg/kg body weight of Etp-sol or Etp-oil. The tissue distribution of etoposide was subsequently analyzed by high performance liquid chromatography. Compared with Etp-sol, Etp-oil delivered significantly greater amounts of etoposide and for a longer period to the omentum, taken as representative of the intraperitoneal tissue, and the etoposide concentration in blood plasma was increased more slowly and decreased more gradually.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1432-0843
    Keywords: Etoposide ; Toxicity ; Intraperitoneal administration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We compared the safety and efficacy in mice with peritoneal carcinomatosis of two etoposide formulations: an aqueous solution (Etp-sol) and particles suspended in oil (the addition products of iodine and the ethyl esters of the fatty acids obtained from poppy-seed oil (Lipiodol) or sesame oil; Etp-oil). We also investigated tissue distribution of etoposide in rats treated with Etp-oil and Etp-sol. Etoposide was injected intraperitoneally at concentrations ranging from 52 to 392 mg/kg (increasing geometrically by a factor of 1.4). The 50% lethal dose (LD50), determined over a 2-week period of observation, was 135 mg/kg for Etp-oil and 108 mg/kg for Etp-sol. Autopsy findings included macroscopic intestinal bleeding, necrosis of the intestinal mucosa, and pulmonary congestion in mice from both treatment groups. In the efficacy trials. 106 P388 leukemia cells were transplanted into CDF1 male mice, and Etp-oil and Etp-sol were injected at doses of 20 mg/kg and 80 mg/kg. In the groups receiving the 20 mg/kg dose, 11 of 19 mice in the Etp-oil group survived to day 60 compared with 3 of 20 mice in the Etp-sol group. Toxicity-related deaths occurred in 1 of 20 mice treated with 80 mg/kg Etp-oil and in 8 of 20 mice treated with 80 mg/kg Etp-sol. No cancer-related deaths were associated with the 80 mg/kg dose in either treatment group. Our findings showed that the Etp-oil was associated with a lower toxicity and a higher efficacy than the Etp-sol. To evaluate tissue distribution, rats were injected intraperitoneally with 5 mg/kg body weight of Etp-sol or Etp-oil. The tissue distribution of etoposide was subsequently analyzed by high performance liquid chromatography. Compared with Etp-sol, Etp-oil delivered significantly greater amounts of etoposide and for a longer period to the omentum, taken as representative of the intraperitoneal tissue, and the etoposide concentration in blood plasma was increased more slowly and decreased more gradually.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1432-041X
    Keywords: Key words Metalloproteinase ; Hydra ; Extracellular matrix ; Axial patterning ; Peptides
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Peptides serve as important signaling molecules in development and differentiation in Hydra. Two peptides, Hym-346 and pedibin, have recently been identified to act as morphogenetic signals for foot differentiation. In screening for target genes for Hym-346 we have isolated an astacin matrix metalloprotease, termed foot activator responsive matrix metalloprotease (Farm1). Farm1 is normally expressed in epithelial cells of the gastric region and absent in apical and basal tissue. Incubation of polyps in peptides Hym-346/pedibin causes immediate downregulation of Farm1 expression. A structurally unrelated peptide, Hym-323, which also enhances foot formation in Hydra, also downregulates Farm1 expression. Treatment of polyps with the ectopic feet-inducing agent LiCl also resulted in decreased level of Farm1 transcripts. Thus metalloproteinase Farm1 is a transcriptional target of positional signals specifying foot differentiation and appears to play a potent role in basal patterning processes.
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