ISSN:
1750-3841
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Tomato paste carotenoids were separated by gradient-elution normal-phase open-column chromatography and analyzed by isocratic-elution reverse-phase HPLC with UV-VIS photodiode array detection. The normal-phase elution order of the trans pigments was phytoene, phytofluene, beta-, zeta-, gamma-carotene and lycopene. The four dominant tomato paste carotenoids (550 ppm), in increasing quantities, were phytofluene, beta-carotene, phytoene and lycopene. Simultaneous multi-channel monitoring and rapid spectral scans allowed the detection of cis-isomers (e.g., lycopene, phytofluene and beta-carotene) and minor carotenoid components (e.g., alpha-carotene, lycoxanthin and cis-mutatoxanthin). Presentation of results in isograms (wavelength versus retention time) and an extraction scheme will be discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-2621.1988.tb08994.x
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