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  • 1
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Haploinsufficiency of Dll4, a vascular-specific Notch ligand, has shown that it is essential for embryonic vascular development and arteriogenesis. Mechanistically, it is unclear how the Dll4-mediated Notch pathway contributes to complex vascular processes that demand meticulous coordination ...
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 329 (1985), S. 1-8 
    ISSN: 1432-1912
    Keywords: Cardenolides ; Noradrenaline release ; Calcium ; Neurotransmission ; Na,K-ATPase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 1. The requirement for external Ca (Cao) of neurotransmitter release evoked by cardenolides has been investigated in canine saphenous vein. 2. Basal efflux of3H-compounds from saphenous veins pre-loaded with3H-noradrenaline was the same in the absence as in the presence of Cao; Cao is not required for basal efflux of neurotransmitter. 3. Efflux of3H-compounds was increased by cardenolides. Both ACS and ouabain caused a similar maximum net efflux of3H suggesting that each evokes release from the same pool of3H compounds. 4. The similarity of the effects obtained with cardenolides to those obtained during exposure of saphenous vein preparations to potassium-free media suggests that3H-efflux is the result of Na, K-ATPase inhibition. 5. With ACS (ca. EC50) the net efflux of3H-compounds early (〈60 min) in the release period was greater in the absence of Cao than in its presence whereas at longer times the reverse was true; net efflux was less in the absence of Cao than in its presence. The difference in the3H-efflux pattern was paralleled qualitatively throughout by efflux of3H-noradrenaline. 6. The ACS-evoked efflux of3H-compounds in the presence and absence of Cao derives from sympathetic, noradrenergic nerves;3H present in extraneuronal tissues was not released by the cardenolide. 7. With ouabain (〈EC50) the total efflux of3H over a 75 min period was greater in the absence of Cao than in its presence. The reverse was found with ouabain (〉EC50): the total efflux of3H was less in the absence of Cao than in its presence. 8. In the presence of Cao the Ca-channel blocker VP reduced the total3H-efflux evoked by ouabain (〉EC50), but did not reduce that evoked by ouabain (〈EC50). 9. It is concluded that cardenolide evoked neurotransmitter efflux resulting from Na,K-ATPase inhibition is not dependent upon Cao although its presence, and subsequent entry via VP sensitive channels, does increase the magnitude of the efflux caused by higher concentrations of cardenolides. Whether a component of that efflux evoked by lower concentrations of cardenolides, (or higher concentrations over short time periods) depends on internal Ca stores or is independent of the cation, is not yet clear.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1573-174X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Nature of Science, Research, Systems of Higher Education, Museum Science
    Notes: Abstract A class of 1st-year medical students was divided into a control group who performed a laboratory experiment for themselves, and an experimental group who were given a demonstrationdata interpretation exercise. Prior to the practical session the experimental group were given a programmed text covering the underlying theoretical principles, to use in their private study. The control group were instructed to do their private study in the usual way using only their lecture notes and textbooks. Prior knowledge and the gain resulting from practical instruction were assessed using pre- and post-tests. The conventional laboratory method was ineffective and did not facilitate conceptual understanding. The post-test performance and gain of the experimental group was significantly better than that of the control group, but this result did not appear to be dependent upon greater prior knowledge. The pretest performance of both treatment groups revealed that many students were inadequately prepared for the practical exercise. Many students did not appear to study the programmed text conscientiously, probably because they were accustomed to the traditional method of teaching and adopted a surface, reproductive approach to all learning. Furthermore, the students had difficulty linking their prior knowledge to the experiment, suggesting the need for pre-laboratory exercises designed to achieve closer integration between theory and experimentation. It is clear that in order to achieve more meaningful learning there is a need for the students to change their attitudes and approaches to learning and for active, student-centred teaching-learning experiences to be incorporated into the curriculum.
    Type of Medium: Electronic Resource
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