ISSN:
1573-5028
Keywords:
Lilium longiflorum
;
Freesia refracta
;
Tulipa gesneriana
;
GUS mRNA
;
particle bombardment
;
pollen
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Gold particles coated with β-glucuronidase (GUS) mRNA with a 5′ cap structure that had been synthesized in vitro were introduced, by use of a pneumatic particle gun, into pollen grains of lily (Lilium longiflorum), freesia (Freesia refracta) and tulip (Tulipa gesneriana). A fluorometric assay for the GUS activity indicated that in vitro synthesized GUS mRNA introduced into these pollen cells by particle bombardment was successfully expressed. GUS activity in extracts of the bombarded lily pollen became detectable fluorometrically within 30 min after bombardment, peaked at 6 h, then gradually decreased. This activity changed as a function of the developmental stage of the pollen cell of lily.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00020252
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