ISSN:
1524-475X
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
Hydration of keratinocytes modifies the levels of cytokines they secrete, which in turn impacts the secretory behaviour of dermal fibroblasts. In an in vitro coculture model, conditioned media (CM) collagen content was decreased 44% when keratinocytes were hydrated. We hypothesized that this is partly due to increased MMP-activity. We used the same coculture model to study changes in MMP-activity and TIMP secreted by keratinocytes as well as by fibroblasts in monoculture and in coculture in relation to air-treatment or hydration of keratinocytes. Stratified human epidermal keratinocytes (HEK) and confluent human dermal fibroblasts (HDF) were cocultured for 72 h under serum-free conditions. HEK were either kept at the air-interface or hydrated. CM was assayed for MMP-1, −2, −9, TIMP-1 and −2 were assayed using zymograms, western blotting, and ELISA. MMP-1, secreted by both cell types, increased significantly in cocultures compared to monocultures (4-fold in the air-treated group, 26-fold in the hydrated group). MMP-2, secreted mainly by HDFs, was significantly increased by coculture (hydration: 2.4-fold, air: 2.8-fold). MMP-9, predominantly secreted by air-treated HEKs and was significantly decreased in hydrated monoculture (76%) and coculture. HEK-monoculture hydration also significant decreased MMP-1 (86%) and MMP-2 (81%) activity. HDF-secreted TIMP-1 expression was significantly increased by coculture and was unaffected by hydration. Our findings demonstrate that paracrine interactions between HEK and HDF modify MMP activity and that HEK hydration significantly effects on MMP activity. The findings provide insight into the role of hydration on HEK and HDF ctivity during the wound healing process.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1067-1927.2004.0abstractxp.x
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