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  • 1
    Electronic Resource
    Electronic Resource
    A human B lymphocyte antigen (P-76) shared by B-cell chronic lymphocytic leukemia cells and hairy cell leukemia cells (1983)
    Springer
    Annals of hematology 46 (1983), S. 249-259 
    Details
    ISSN: 1432-0584
    Keywords: B-lymphocyte ; Chronic lymphocytic leukemia ; Hairy cell leukemia ; Antiserum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A membrane antigen with an apparent specificity to B lymphocytes was detected with immunochemical techniques and its properties were analyzed. Anti-B-CLL serum was raised in a rabbit by immunization with B-cell chronic lymphocytic leukemia (B-CLL) cells. This anti-B-CLL serum was absorbed with erythrocytes, liver homogenate and insolubilized immunoglobulins. After further absorption with T-CLL cells, chronic myelocytic leukemia (CML) cells and acute myelocytic leukemia (AML) cells, the anti-B-CLL serum still reacted with peripheral blood B lymphocytes, B-CLL cells and hairy cell leukemia (HCL) cells. In contrast, no reactivity was seen with peripheral blood T lymphocyte or monocytes, or leukemia cells of non-B cell origin. An immunoprecipitation of radiolabeled cell surface proteins was attempted using the anti-B-CLL serum in the presence of Staphylococcus Aureus Cowan 1 (SaCl), and the precipitates were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). A membrane antigen with an apparent molecular weight of 76,000 daltons (P-76) was immunoprecipitated with the anti-B-CLL serum from the lysates of normal B lymphocyte, B-CLL cells and HCL cells. The antigen (P-76) is not composed of disulfide-linked subunits and has no structural relationship with HLA-DR (Ia-like) antigens or other known antigens. These results suggest that this antigen is B-lymphocyte specific, and favour the B-lymphocyte nature of HCL cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00319865
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  • 2
    Electronic Resource
    Electronic Resource
    Ultrastructural analysis of membrane-bound polysomes in mouse “nonsecretory” myeloma (nonproducing type) (1983)
    Springer
    Annals of hematology 47 (1983), S. 147-152 
    Details
    ISSN: 1432-0584
    Keywords: Mouse myeloma ; Immunoglobulin ; Membrane-bound polysome ; Ultrastructural analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Using our electron microscopic method of polysome analysis we ascertained the numbers of membrane-bound polysomes and their constituent ribosomes in myeloma cells of J 606 (IgG3) and MOPC315 (IgA) parent cell lines and in their variants that did not produce and, accordingly, secrete either H- or L-chains. Both variants had far fewer membrane-bound polysomes than the respective parent lines. The polysome distribution curve drawn for each of these variants showed one peak at 5 – 6 ribosomes. In contrast to this finding, the distribution curves for the J 606 and MOPC315 parent lines gave two peaks. These observations on mouse myelomas strongly resembled those on human myelomas.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00320176
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Heavy chain loss after treatment with melphalan in a patient with “nonsecretory” myeloma (1983)
    Springer
    Annals of hematology 46 (1983), S. 73-80 
    Details
    ISSN: 1432-0584
    Keywords: Multiple myeloma-Plasma cell ; Electron microscopy ; Fluorescent antibody technique
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A case of “nonsecretory” myeloma is described. The patient had typical osteolytic lesions and marked infiltration of myeloma cells in the bone marrow, and plasma cell leukemia. A good partial remission was obtained with Melphalan, but the patient relapsed and died one year later. Immunofluorescent and immunoelectroscopic studies on the myeloma cells demonstrated the presence of cytoplasmic γ-and κ-chains at the initial stage and of only κ-chains at a relapse. The electron microscopic method for polysome analysis indicated that both L-and H-chains were synthesized on membrane-bound polysomes initially, but the ability to produce H-chain was missing at the relapse.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00320661
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  • 4
    Electronic Resource
    Electronic Resource
    Immunoglobulin production and secretion in bence jones protein myeloma and “nonsecretory” myeloma (1982)
    Springer
    Annals of hematology 45 (1982), S. 121-129 
    Details
    ISSN: 1432-0584
    Keywords: Multiple myeloma ; Immunoglobulin ; Electron microscopy ; Fluorescent antibody technique
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Using our electron microscopic method for polysome analysis and an immunofluorescent technique we studied Ig production and secretion by tumor cells in seven BJP myeloma patients and seven “nonsecretory” myeloma patients. In BJP myeloma Ig production and secretion is of three types: Type 1, only L-chains are synthesized and secreted; Type 2, the myeloma cells show fluorescence for H-chains, but upon polysome analysis there is no peak at polysomes corresponding to H-chain production; Type 3, the myeloma cells show fluorescence for H-chains, and polysome analysis shows two peaks corresponding to L- and H-chain production. Polysome analysis of “nonsecretory” myelomas show the presence of only very few membrane-bound polysomes and their distribution curves are entirely different from those of “ordinary” myeloma. Furthermore, the distribution patterns vary among seven cases. Results obtained by polysome analysis and immunofluorescent technique suggest that the “nonsecretory” myeloma could be divided into several subtypes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00319940
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  • 5
    Electronic Resource
    Electronic Resource
    Congenital dyserythropoietic anemia type I: A freeze-fracture and thin section electron microscopic study (1983)
    Springer
    Annals of hematology 46 (1983), S. 329-338 
    Details
    ISSN: 1432-0584
    Keywords: Dyserythropoietic anemia ; Freeze-fracture ; Erythroblast ; Nuclear pore
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Congenital dyserythropoietic anemia type I was diagnosed in a Japanese patient. Electron microscopic examination of thin sections revealed the features of erythroblasts peculiar to CDA type I; namely “spongy appearance” of nucleus and “internuclear chromatin bridge” etc. However, the widening of nuclear pores, which has been reported as another peculiar feature of CDA type I erythroblasts, were not confirmed. Aberrations of erythroblast nuclear membrane which have not been hitherto noticed were as follows: The nuclear evelopes of erythroblasts frequently lacked heterochromatin application in wide areas where they often showed a marked invagination or evagination. This is associated with the invasion of cytoplasmic organelles into the nuclear territory and occasionally with the extrusion of the nucleolus into the cytoplasm. There were no nuclear pores on the invaginated or the evaginated portions of the nuclear envelope where the perinuclear cistern was compressed to lose the cisternal space, or widened to contain some membrane debris. The findings with thin sectionings were confirmed with freeze-fracturing. Both findings suggest that an aberration may reside in the erythroblast nuclear membrane itself, but not in nuclear pores.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00320693
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Localization of cytochrome P-450 in the colonic mucosa of 3-methylcholanthrene-pretreated and untreated rats An immunohistochemical study (1988)
    Springer
    Cell & tissue research 252 (1988), S. 397-401 
    Details
    ISSN: 1432-0878
    Keywords: Colonie mucosal epithelium ; Cytochrome P-450 ; 3-Methylcholanthrene ; 7-Ethoxycoumarin ; Immunohistochemistry ; Western-blotting ; Rat (Wistar)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Immunohistochemical localization of cytochrome P-450 in the colonic mucosa of 3-methylcholanthrene-pretreated and untreated rats was studied by indirect fluorescent antibody staining technique. A polyclonal antibody for cytochrome P-450MC purified from hepatic microsomes of 3-methylcholanthrene-pretreated rats was used for this experiment. A strong immunofluorescence was found to be localized in the cytoplasm of the surface epithelium of the mucosa in the colon of 3-methylcholanthrene-pretreated rats. A faint immunofluorescence was also observed in the epithelium of untreated rats. 7-Ethoxycoumarin O-deethylase activity of colonic microsomes was significantly enhanced by 3-methylcholanthrene-pretreatment in parallel with an increase in the intensity of immunostaining for cytochrome P-450MC in Western blotting analysis. This is the first report on the localization of cytochrome P-450 in the colonic mucosa.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00214382
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