ISSN:
1432-0614
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Abstract The thermophilic bacterium, Thermus species ATCC 27978, which is capable of aerobically degrading benzene, toluene, ethylbenzene, and the xylenes (BTEX), was cultured in 5-1 fermentors on a Castenholz salts-tryptone medium. This bacterium can be cultivated more conveniently at 45 °C, a temperature substantially lower than its optimal growth temperature (approx. 60 °C). Yet, the washed harvested cells from such cultures display the same initial BTEX-degrading activity as those when Thermus sp. is grown at its higher optimal temperature. Two bioreactor cultivation modes, batch and fed batch, were investigated. More biomass and more BTEX-degrading activity (assayed at 60 °C) were generated in fed-batch cultures than in the growth-limited batch cultures. The former yielded a biomass concentration of 2.5 g dry cell weight (DCW) l−1 and whole-cell degrading specific activities of 7.6 ± 1.3, 10.1 ± 1.9, 9.8 ± 2.1, 2.3 ± 0.5, and 4.6 ± 0.9 nmol degraded (mg DCW)−1 min−1 for benzene, toluene, ethylbenzene, m-xylene, and the o- plus p-xylenes (unresolved mixture), respectively. Although the formation of cellular BTEX-degrading activity is growth-associated, a slow to moderate specific growth rate of 0.02–0.07 h−1 favors the production of BTEX-degrading activity, while a high growth rate, of the order of 0.16 h−1, is detrimental to its production. The washed harvested Thermus sp. cells were capable of degrading BTEX over a broad range of thermophilic incubation temperatures, 45–77 °C.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002530051002
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