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  • 1
    Electronic Resource
    Electronic Resource
    Molecular cloning and functional characterization of a novel delayed rectifier potassium channel from channel catfish (Ictalurus punctatus): expression in taste buds (2001)
    Oxford, UK : Blackwell Science Ltd
    Journal of neurochemistry 76 (2001), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The gustatory system of channel catfish is widely studied for its sensitivity to amino acids. As a first step in identifying the molecular components that play a role in taste transduction in catfish, we cloned the full-length cDNA for Kv2-catfish, a novel K+ channel that is expressed in taste buds. The deduced amino acid sequence is 816 residues, and shares a 56–59% sequence identity with Kv2.1 and Kv2.2, the other members of the vertebrate Kv2 subfamily of voltage-gated K+ channels. The Kv2-catfish RNA was expressed in taste buds, brain, skeletal muscle, kidney, intestine and gills, and its gene is represented as a single copy in the catfish genome. Recombinant channels expressed in Xenopus oocytes were selective for K+, and were inhibited by tetraethylammonium applied to the extracellular side of the membrane during two-electrode voltage clamp analysis with a 50% inhibitory constant of 6.1 mm. The channels showed voltage-dependent activation, and did not inactivate within 200 ms. Functionally, Kv2-catfish is a voltage-gated, delayed rectifier K+ channel, and its primary structure is the most divergent sequence identified among the vertebrate members of the Kv2 subfamily of K+ channels, being related equally well to Kv2.1 and Kv2.2.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1471-4159.2001.00137.x
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  • 2
    Electronic Resource
    Electronic Resource
    Reproductive Pheromones of the Landlocked Sea Lamprey (Petromyzon marinus) (1987)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 510 (1987), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1987.tb43485.x
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  • 3
    Electronic Resource
    Electronic Resource
    Spatial gradient detection of chemical cues by catfish (1980)
    Springer
    Journal of comparative physiology 140 (1980), S. 95-99 
    Details
    ISSN: 1432-1351
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary 1. Unrestrained brown bullhead catfish (Ictalurus nebulosus) were trained to turn toward the higher concentration when two concentrations of a mixture of amino acids were simultaneously flowed over the cutaneous taste epithelium on the right and left maxillary barbels. 2. The minimum detectable Weber fraction was dependent upon the stimulus solution concentration with smaller Weber fractions being detected at weaker concentrations. Brown bullheads were capable of detecting a concentration difference of 0.3 Weber fractions at the highest stimulus solution concentration and 0.1 Weber fractions at the lowest. 3. Variations in the time-of-arrival of the stimuli at the two barbels, greater than those occurring during normal testing, did not alter the values of the minimum discernable Weber fraction. This indicates that spatial rather than temporal differences in concentration were the basis of these responses. 4. Anosmic bullheads were as sensitive to concentration differences as intact fish, indicating that discrimination was made using the taste receptors on the barbels. 5. These experiments show that bullheads can compare the intensity of a taste stimulus at two distinct receptor fields and thus define a gustatory chemical space. This ability is a requirement for tropotactic orientation to chemical gradients and suggests that such a mechanism may be important in nature.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00606301
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  • 4
    Electronic Resource
    Electronic Resource
    IP3-and cAMP-induced responses in isolated olfactory receptor neurons from the channel catfish (1992)
    Springer
    The journal of membrane biology 127 (1992), S. 173-183 
    Details
    ISSN: 1432-1424
    Keywords: olfactory receptor neuron ; IP3 ; cAMP olfactory ; transduction ; second messengers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary Olfactory receptor neurons enzymatically dissociated from channel catfish olfactory epithelium were depolarized transiently following dialysis of IP3 or cAMP (added to the patch pipette) into the cytoplasm. Voltage and current responses to IP3 were blocked by ruthenium red, a blocker of an IP3-gated Ca2+-release channel in sarcoplasmic reticulum. In contrast, the responses to cAMP were not blocked by extracellularly applied ruthenium red, nor by l-cis-diltiazem or amiloride and two of its derivatives. The current elicited by cytoplasmic IP3 in neurons under voltage clamp displayed a voltage dependence different from that of the cAMP response which showed marked outward rectification. A sustained depolarization was caused by increased cytoplasmic IP3 or cAMP when the buffering capacity for Ca2+ of the pipette solution was increased, when extracellular Ca2+ was removed or after addition of 20–200 nm charibdotoxin to the bathing solution, indicating that the repolarization was caused by an increase in [Ca i ] that opened Ca2+-activated K+ channels. The results suggest that different conductances modulated by either IP3 or cAMP are involved in mediating olfactory transduction in catfish olfactory receptor neurons and that Ca2+-activated K+ channels contribute to the termination of the IP3 and cAMP responses.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00231505
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  • 5
    Electronic Resource
    Electronic Resource
    Sex pheromones of the sea lamprey (Petromyzon marinus): Steroid studies (1987)
    Springer
    Journal of chemical ecology 13 (1987), S. 387-395 
    Details
    ISSN: 1573-1561
    Keywords: Sea lamprey ; Petromyzon marinus ; pheromone ; urine ; steroids ; radioimmunoassay ; RIA ; behavior
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Pheromone-containing and pheromone-devoid samples of male sea lamprey (Petromyzon marinus) urine were analyzed for the concentrations of nine steroids [dehydroepiandrosterone (DHEA), testosterone (T), dihydrotestosterone (DHT), progesterone (P), androstenedione (A), estrone (E1), estradiol (E2), corticosterone (B), and cortisol (F)] by radioimmunoassay (RIA). Samples analyzed included native urine that had been enzymatically hydrolyzed with mixed β-glucuronidase/sulfatase. Values of the analyses were used to prepare solutions of the individual steroids for bioassay at concentrations which bracketed the urinary concentrations. Results show that only testosterone elicited a preference response in spawning-run female sea lampreys, and in concentrations three to four orders of magnitude greater than those found in active, unhydrolyzed male urine. The possibility that testosterone acts as a pheromone in this species is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01025898
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