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  • 1
    Electronic Resource
    Electronic Resource
    Isolation of a specific chromosomic DNA sequence of Bacillus anthracis and its possible use in diagnosis (1996)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS immunology and medical microbiology 15 (1996), S. 0 
    Details
    ISSN: 1574-695X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Abstract A 277-bp long DNA fragment, Ba813, was isolated from an avirulent Bacillus anthracis strain 7700 genomic library. Two oligonucleotides derived from the Ba813 sequence were used as primers in polymerase chain reaction tests on genomic DNA from 28 Bacillus anthracis and from 33 heterologous bacteria strains. A specific, 152-bp long DNA fragment was amplified only when Bacillus anthracis DNA was used as the target. The amplified product was analysed by non-radioactive sandwich hybridisation in microtiter plates using two oligonucleotides. The capture oligonucleotide C1 was covalently linked onto aminated wells of microtiter plates. The detection oligonucleotide D3 was labelled with biotine. The hybrid molecules were detected by avidine conjugated with alkaline phosphatase and chromogenic substrate. Amplification of Ba813 sequence may provide the basis for rapid and reliable assay for the detection and identification of Bacillus anthracis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-695X.1996.tb00088.x
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Biological activity of functionalized SiO2 thin films prepared by sol-gel method (1996)
    Springer
    Journal of sol gel science and technology 7 (1996), S. 81-85 
    Details
    ISSN: 1573-4846
    Keywords: sol-gel ; functionalized films ; grafting of antibodies ; biological activity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract In this work we investigated the biological properties of sol-gel films in aqueous medium. Functionalized silica films were prepared by the sol-gel process, from organically modified silicon alkoxides with amino or thiol groups. Covalent binding of proteins with different orientations according to the hydrophilic or hydrophobic character of the surface was studied. This binding occurred via a covalent coupling agent providing a very stable linkage. No denaturation was detected and a good detection of the antigen was observed. Immunoassays have demonstrated the biological activity of grafted antibodies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00401887
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Quantitation of polymerase chain reaction-amplified DNA fragments by capillary electrophoresis and laserinduced fluorescence detection (1996)
    Weinheim : Wiley-Blackwell
    Electrophoresis 17 (1996), S. 512-517 
    Details
    ISSN: 0173-0835
    Keywords: DNA fragments ; Quantitative competitive polymerase chain reaction ; Capillary electrophoresis ; Fluorescence detection ; Bacillus anthracis ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Quantitation of DNA fragments amplified by polymerase chain reaction (PCR) is needed for the determination of target DNA in molecular biology. Capillary electrophoresis in entangled polymer solution coupled to laser-induced fluorescence detection was assessed as an alternative technique to conventional slab gel methods to monitor competitive PCR, which consists of amplifying an internal standard fragment under the same conditions as the target fragment. The fluorescence signal was generated either through end-labeling of the fragments using 5′-fluorescein-labeled primers or through intercalation of ethidium bromide along the double strand. It is shown that the more accurate and reliable results were obtained using this latter pathway.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150170316
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    Paper (German National Licenses)
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