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  • 1
    ISSN: 1434-0879
    Keywords: COM crystal growth ; Pyrophosphate ; Citrate ; Rat urine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary An assay system for the measurement of the rate of Calcium Oxalate Monohydrate (COM) seed crystal growth in a metastable solution of calcium chloride and sodium oxalate containing traces of 14C-oxalic acid was used to assess the inhibitory activity of pyrophosphate (10−5 M-10−4 M), citrate (10−4 M-10−3 M) and urines of normal and pyridoxine deficient rats. Both pyrophosphate and citrate were strong inhibitors of COM crystal growth and caused a 50% decrease in crystal growth rate at 1.50×10−5 M and 2.85×10−4 M respectively. Normal rat urine strongly inhibited the COM crystal growth, while pyridoxine deficient animals showed a significant (p〈 0.01) decrease in mean inhibitory activity as compared to pair-fed controls. A lowered urinary inhibitory potential accompanied with hyperoxaluria and hypercalciuria, which is known to be associated with pyridoxine deficiency, may be a contributory risk of calcium oxalate crystallization and stone formation.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1434-0879
    Keywords: Magnesium oxide ; Pyridoxine ; Calcium oxalate ; Urolithiasis ; Therapeutic effect
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A combined supplement of magnesium oxide (300 mg/day) and pyridoxine·HCl (10 mg/day) was given p.o. to 16 recurrent calcium oxalate (CaOx) stone formers, and its therapeutic efficacy was biochemically evaluated by measuring various parameters of blood (Na, K, Mg, urea, creatinine, calcium, phosphate, uric acid, alanine transaminase, aspartate transaminase and alkaline phosphatase) and urine (volume, pH, creatinine, Na, K, Mg, uric acid, calcium, phosphate, oxalate and citrate) at 0, 30, 60, 90 and 120 days of treatment. Serum Mg significantly (P〈0.01) increased after 30 days of treatment and remained constant thereafter while other blood parameters were unaltered. Combined treatment led to a significant increase in the urinary excretion of Mg and citrate over pretreatment values while oxalate excretion showed a gradual and significant decline during the therapy. The results confirmed the efficacy of MgO-pyridoxine supplementation in terms of changes in urinary excretion of lithogenic and inhibitory components, leading to a significant (P〈0.01) decrease in CaOx risk index from 0.09±0.04 at 0 day to 0.05±0.02 after 120 days of treatment.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1434-0879
    Keywords: Urinary calculi ; Citrate ; Circadian rhythm
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Urinary citrate excretion was estimated colorimetrically from urine samples collected every 3 h for 24 h from 25 healthy adult males (non-stone formers; mean age 39±7 years) and 25 male patients suffering from calcium nephrolithiasis (stone formers; mean age 41±6 years). The 24 h citrate excretion was 2.47±0.65 mmol in non-stone formers and 2.02±0.71 mmol in stone formers. This difference was not significant. However, cosinor rhythmometry revealed a significant circadian rhythmicity in urinary citrate excretion in the healthy males which was absent in the stone formers; the amplitude was 0.06 mmol in non-stone formers and 0.017 mmol in stone formers. The acrophase was located at 14:25 h in non-stone formers and at 23:30 h in stone formers.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1573-5087
    Keywords: moisture stress ; polyethylene glycol ; long-chain aliphatic alcohols ; seedling growth, solute accumulation ; hydrolases ; wheat ; Triticum aestivum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Polyethylene glycol (PEG)-induced water stress adversely affected the germination and seedling growth of Sonalika and WL2265 cultivars of wheat (Triticum aestivum L.). Application of a mixture of long-chain aliphatic alcohols having the composition C-24 Tetracosanol (10%), C-26 Hexacosanol (16%), C-28 Octacosanol (15%), C-30 Tricontanol (30%), C-32 Dotriacontanol (15%) and C-34 Tetratriacontanol (14%), partially ameliorated these effects and promoted both percent germination and seedling growth. Application also stimulated the activities of the hydrolases α- and β-amylase and acid invertase, so increasing free sugar accumulation. A role for long chain aliphatic alcohols in the regulation of carbohydrate metabolism is suggested. The alleviation of moisture stress by application of this mixture suggest that long chain aliphatic alcohols may be most effective at low water potentials.
    Type of Medium: Electronic Resource
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