Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Expression of the green fluorescent protein gene in conifer tissues (1997)
    Springer
    Plant cell reports 16 (1997), S. 267-271 
    Details
    ISSN: 1432-203X
    Keywords: Abbreviations: GUS, β-glucuronidase; NOS, nopaline synthase; NPTII, neomycin phosphotransferase; CaMV, cauliflower mosaic virus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. The gene coding for the green fluorescent protein (GFP) from jellyfish was introduced into conifer tissues by microprojectile bombardment and its transient expression was detected. Two versions of the GFP gene, wild-type GFP and modified GFP with a cryptic intron removed, were directly compared for their expression in black spruce pollen. While the wild-type GFP gene resulted in a low level of expression, the modified GFP gene gave a dramatic increase in amount of expression (〉100 times). The expression of GFP was detected in all the tissues tested: pollen, embryonal masses, suspension culture, and somatic embryos. Also, the GFP gene was introduced and expressed in three different conifer species (black spruce, white spruce, and white pine). The successful expression of the GFP gene in various tissues and different species suggests that it will be a useful reporter/marker gene for conifers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01088278
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Expression of the green fluorescent protein gene in conifer tissues (1997)
    Springer
    Plant cell reports 16 (1997), S. 267-271 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The gene coding for the green fluorescent protein (GFP) from jellyfish was introduced into conifer tissues by microprojectile bombardment and its transient expression was detected. Two versions of the GFP gene, wild-type GFP and modified GFP with a cryptic intron removed, were directly compared for their expression in black spruce pollen. While the wild-type GFP gene resulted in a low level of expression, the modified GFP gene gave a dramatic increase in amount of expression (〉100 times). The expression of GFP was detected in all the tissues tested : pollen, embryonal masses, suspension culture, and somatic embryos. Also, the GFP gene was introduced and expressed in three different conifer species (black spruce, white spruce, and white pine). The successful expression of the GFP gene in various tissues and different species suggests that it will be a useful reporter/marker gene for conifers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01088278
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Functional analysis of a RPD3 histone deacetylase homologue in Arabidopsis thaliana (2000)
    Springer
    Plant molecular biology 44 (2000), S. 167-176 
    Details
    ISSN: 1573-5028
    Keywords: Arabidopsis ; gene repression ; histone deacetylase ; RPD3
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Histone acetylation is modulated through the action of histone acetyltransferase and deacetylase, which play key roles in the regulation of eukaryotic gene expression. We have screened the expressed sequence tag database with the yeast histone deacetylase RPD3 sequence and identified two Arabidopsis homologues, AtRPD3A and AtRPD3B. The deduced amino acid sequences of AtRPD3A and AtRPD3B show high overall homology (55% identity) to each other. AtRPD3A encodes a putative protein of 502 amino acids with 49% identity to the yeast RPD3. AtRPD3B encodes a putative protein of 471 amino acids and shares 55% amino acid identity with the yeast RPD3. Northern analysis indicated that AtRPD3A was highly expressed in the leaves, stems, flowers and young siliques of Arabidopsis plants, whereas the AtRPD3B transcript was not detected in these organs. An AtRPD3A fusion protein repressed transcription when directed to a promoter driving a reporter gene, indicating a role for AtRPD3A protein in gene repression. Arabidopsis plants were transformed with a gene construct comprising a truncated AtRPD3A cDNA in the antisense orientation driven by a strong constitutive promoter, −394tCUP. Antisense expression of AtRPD3A resulted in decreased endogenous AtRPD3A transcript and delayed flowering in transgenic Arabidopsis plants, suggesting that the transition from the vegetative to reproductive phase of development could be affected by histone acetylation. Our study demonstrates the important role of histone deacetylases in plant growth and development.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006498413543
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Development of a simple particle bombardment device for gene transfer into plant cells (1994)
    Springer
    Plant cell, tissue and organ culture 37 (1994), S. 47-53 
    Details
    ISSN: 1573-5044
    Keywords: transient gene expression ; β-glucuronidase ; alfalfa ; soybean ; DNA transfer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A simple particle bombardment device was designed, constructed and shown to be efficient for the delivery of DNA into plant cells. High levels of transient β-glucuronidase expression were observed in alfalfa suspension-cultured cells and embryogenic soybean suspension-cultured cells. Expression of β-glucuronidase in alfalfa suspension-cultured cells was used to optimize the bombardment conditions for the device. Transient gene expression in alfalfa was found to be dependent on the state of the target tissue, the size of particles employed, the helium pressure used to accelerate the particles and the distance travel led by the tungsten particles carrying DNA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00048116
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...