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  • 1
    Electronic Resource
    Electronic Resource
    Regulation of muscarinic ligand binding sites by nerve growth factor in PC12 phaeochromocytoma cells (1982)
    [s.l.] : Nature Publishing Group
    Nature 297 (1982), S. 152-154 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] PC12 cells (passages 23-32) were derived from the clone originally isolated by Greene and Tischler1. Cells were cultured in collagen-coated 10-cm polystyrene dishes as described previously1'6. To treat cells with NGF for binding experiments, replicate cultures were inoculated at a density of 4x 104 ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/297152a0
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  • 2
    Electronic Resource
    Electronic Resource
    Pheochromocytoma cell lines from heterozygous neurofibromatosis knockout mice (2000)
    Springer
    Cell & tissue research 302 (2000), S. 309-320 
    Details
    ISSN: 1432-0878
    Keywords: Pheochromocytoma Neurofibromatosis Neurofibromin Phenylethanolamine-N-methyltransferase Transcription Silencing Mouse (knockout mutation of NF1)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Transplantable tumors and cell lines have been developed from pheochromocytomas arising in mice with a heterozygous knockout mutation of the neurofibromatosis gene, Nf1. Nf1 encodes a ras-GTPase-activating protein, neurofibromin, and mouse pheochromocytoma (MPC) cells in primary cultures typically show extensive spontaneous neuronal differentiation that may result from the loss of the remaining wild-type allele and defective regulation of ras signaling. However, all MPC cell lines express neurofibromin, suggesting that preservation of the wild-type allele may be required to permit the propagation of MPC cells in vitro. MPC lines differ from PC12 cells in that they express both endogenous phenylethanolamine N-methyltransferase (PNMT) and full-length PNMT reporter constructs. PNMT expression is increased by dexamethasone and by cell-cell contact in suspension cultures. Mouse pheochromocytomas are a new tool for studying genes and signaling pathways that regulate cell growth and differentiation in adrenal medullary neoplasms and are a unique model for studying the regulation of PNMT expression.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004410000290
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  • 3
    Electronic Resource
    Electronic Resource
    Immunoreactivity of normal rabbit serum with epinephrine (E) cells of the rat adrenal medulla after microwave antigen retrieval (1998)
    Springer
    Cell & tissue research 293 (1998), S. 563-566 
    Details
    ISSN: 1432-0878
    Keywords: Key words Chromaffin cells ; Immunohistochemistry ; Natural antibodies ; Chromogranin ; Secretogranin ; Rat (F344 ; Crl: CDBR)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  Normal rabbit serum (NRS) produces intense staining of epinephrine (E) cells in microwave-heated sections of rat and mouse adrenal gland. This staining is not eliminated by liver adsorption, complement inactivation, high salt buffer, Triton X-100 or dilution in normal goat serum and bovine serum albumin (BSA), suggesting that it may result from specific antigen-antibody interactions. Western blots of adrenal medullary protein probed with NRS reveal several bands. The major band does not correspond to rat chromogranin A, which is a major constituent of E-cell secretory granules. The findings suggest that NRS may contain autoantibodies against a secreted rabbit E-cell protein with a homologous counterpart in rats and mice, and that this protein may be immunologically unmasked in situ by microwave heating. This phenomenon is a potential source of error in immunohistochemical studies of the adrenal medulla, and has potential biological significance in neuroimmunology.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004410051148
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