ISSN:
1432-1912
Keywords:
ITC-1 cell
;
Low-threshold and transient Ca2+ (T-type) current
;
High-threshold and long-lasting Ca2+ (L-type) current
;
5,5-diphenylhydantoin (phenytoin)
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Transmembrane Ca2+ currents were investigated by means of a whole-cell clamp technique in a hamster glucagon-secreting tumor cell line (ITC-1). Two types of Ca2+ current were identified in ITC-1 cells. The low-threshold and transient (T-type) current became detectable above the potential level around −60 mV and decayed rapidly with an inactivation time constant of 95 ms (at −40 mV and 23°C), while the high-threshold and long-lasting (L-type) one was activated by depolarization more positive to −30 mV with non-inactivating kinetics. The voltage dependence and kinetics of these currents were identical to those reported in guinea-pig pancreatic α2 cells. Both currents were augmented by equimolar substitution of Ca2+ with Ba2+ and completely abolished by adding 1 μM La3+. Phenytoin, a well known anti-epileptic drug and a postulated T-type specific Ca2+ current antagonist, surprisingly blocked the L-type current without affecting the T-type current in ITC-1 cells. While phenytoin antagonized the L-type Ba2+ current selectively, 60% of the current remained even in supramaximal concentration range over 500 μM. The residual component of the L-type current was completely abolished by adding nifedipine.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00175473
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