ISSN:
1615-6102
Keywords:
Agrobacterium rhizogenes
;
rolD promoter
;
GUS gene
;
Nicotiana tabacum
;
Development
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary TherolD gene fromAgrobacterium rhizogenes has recently been shown to induce striking precocity of flowering in transgenic tobacco. A transcriptional fusion between 578 bp of therolD upstream regulating sequence and the GUS reporter gene has been transferred to tobacco plants and its expression analysed throughout the whole life cycle of the plant. A detailed histological analysis revealed thatrolD is strongly expressed in elongating and differentiating tissues of each organ, from the mature embryo to the adult plant. In organs with determinate growth, such as cotyledons, leaves and floral parts,rolD expression follows the age gradient of the tissues. In mature tissues,rolD expression is high, begins to decrease with ageing and is switched off when senescence occurs.RolD is also active in the vascular system, in the procambium, pith- and receptacular-meristems. Our data show that expression ofrolD is under developmental control as it correlates with, and may represent a molecular marker for, the elongation/expansion and maturation phases of the plant tissues.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01279889
Permalink