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  • 1
    Electronic Resource
    Electronic Resource
    Regulation of α3 Nicotinic Acetylcholine Receptor Subunit mRNA Levels by Nerve Growth Factor and Cyclic AMP in PC12 Cells (2000)
    Oxford UK : Blackwell Science Ltd.
    Journal of neurochemistry 74 (2000), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: To investigate the effects of nerve growth factor (NGF)and cyclic AMP (cAMP) on the level of the nicotinic acetylcholine receptorsubunit α3 mRNA, we used PC12h cells, PC12 cells expressingdominant-negative Ras protein, and the parental PC12 cells. PC12h cells haveNGF-responsive tyrosine hydroxylase activity. Expression of dominant-negativeRas protein prevents the signaling through the Ras-mitogen-activated proteinkinase cascade. The morphological changes of the parental PC12 cells inresponse to NGF and 8-(4-chlorophenylthio)adenosine 3′,5′-cyclicmonophosphate (CPTcAMP), a cell-penetrating cAMP analogue, were similar tothose of PC12h cells. NGF up-regulated the α3 mRNA level in PC12h cellsand down-regulated the α3 mRNA level in the parental PC12 cells.Expression of dominant-negative Ras protein and an inhibitor ofmitogen-activated protein kinase kinase inhibited the effects of NGF onα3 mRNA level. CPTcAMP down-regulated the α3 mRNA level in allthree PC12 cell lines. An inhibitor of protein kinase A inhibited theCPTcAMP-induced down-regulation of α3 mRNA. The α3 mRNAdown-regulation required prolonged treatment with CPTcAMP even after cAMPresponse element binding protein phosphorylation was decreased. Membranedepolarization with high K+ had no effect on the α3 mRNA level in PC12h cells. Based on these results, we propose that at least two unknown effectors regulate α3 mRNA levels in PC12 cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1471-4159.2000.0741346.x
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  • 2
    Electronic Resource
    Electronic Resource
    Gibberellin-induced elongation and osmoregulation in internodes of floating rice (1997)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 99 (1997), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Internodal elongation in floating rice (Oryza sativa L. cv. Habiganj Aman II) is known to be enhanced by treatment with ethylene or gibberellic acid (GA3) at high relative humidity (RH). However, ethylene-induced internodal elongation is inhibited at low RH. while GA3-induced internodal elongation is hardly affected by humidity. We examined the possible involvement of osmoregulation in the stimulation by GA3 of the elongation of internodes at low RH. Submergence and treatment with ethylene or GA33 at 100% RH increased the osmotic potential in internodes of excised stem segments, while GA3 at 20% RH maintained the osmotic potential at a low level. In internodes of stem segments that had been treated with GA3 at 20% RH, the activity of invertase and the level of soluble sugars were almost 2- and 1.5-fold higher, respectively, than those in internodes that had been treated with GA3 at 100% RH. These results indicate that one of the possible mechanisms by which GA3 promotes elongation of internodes at low RH involves the osmoregulation that is achieved by promotion of the synthesis of invertase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1997.tb05351.x
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  • 3
    Electronic Resource
    Electronic Resource
    Relationship of (CAG)nC configuration to repeat instability of the Machado-Joseph disease gene (1996)
    Springer
    Human genetics 〈Berlin〉 98 (1996), S. 643-645 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The mutation responsible for Machado-Joseph disease (MJD) has been identified as an expansion of a CAG trinucleotide repeat in a novel gene on chromosome 14q32.1. The CAG repeat tract is followed by C or G, and alleles are thereby divided into two types on the basis of molecular configuration, (CAG)nC and (CAG)nG. We have studied the relationship between the repeat length and the configuration in 38 patients from 28 Japanese families with MJD, and 31 unrelated normal Japanese subjects. The CAG repeat length in 100 normal alleles ranged from 13 to 37 repeats, while 38 MJD patients had one expanded allele with 64 to 84 repeats. Surprisingly, the expanded alleles had exclusively the (CAG)nC configuration, while both (CAG)nC and (CAG)nG were seen in normal alleles from MJD and control subjects. Furthermore, in normal alleles, the CAG repeat tract was significantly longer in (CAG)nC than in (CAG)nG. These findings suggest that the (CAG)nC configuration is related to repeat instability of the MJD gene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004390050276
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    Paper (German National Licenses)
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