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  • 1
    ISSN: 1365-2036
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background : Host genetic factors may be important in determining not only disease susceptibility, but also disease behaviour and response to therapy in inflammatory bowel disease. Two polymorphisms (C3435T and G2677T/A) of the multidrug resistance 1 gene have been correlated with the altered P-glycoprotein expression and function in humans, and associated with predisposition to ulcerative colitis and Crohn's disease.Aim : To investigate the contribution of these polymorphisms to disease susceptibility and response to medical therapy.Methods : A total of 946 inflammatory bowel disease patients (478 Crohn's disease, 272 males, mean age 43 ± 14 years and 468 ulcerative colitis, 290 males, mean age 48 ± 15 years) and 450 healthy controls were genotyped for the single nucleotide polymorphisms C3435T and G2677T/A. Patients were also classified on the basis of response to medical therapy (mesalazine, steroids, immunosuppressives and infliximab).Results : Both single nucleotide polymorphisms were in Hardy–Weinberg equilibrium and significant linkage disequilibrium. No significant difference in the allele, genotype, and haplotype frequencies was found in both Crohn's disease and ulcerative colitis patients compared with the controls. No correlation with clinical features was found, except for a reduced frequency of extra-intestinal manifestations in Crohn's disease patients with the G2677T genotype (40%) compared with GG2677 and 2677TT genotypes (54% and 58%, respectively) (P = 〈0.02). No significant difference was also found after stratifying the patients on the basis of their response to medical therapy.Conclusion : The investigated polymorphisms of the multidrug resistance 1 gene have no significant role in disease susceptibility and response to medical therapy in our Italian population of inflammatory bowel disease patients.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 46 (1981), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Soft white wheat was sprouted in the laboratory for 1, 2 and 4 days, respectively, before milling the wheat into flours. The flours were used to bake cakes and cookies and to determine thickening powers. The flours milled from wheat which had been sprouted for 2 or 4 days were slightly higher in ash and protein and darker in color than the flour from the sound wheat. Sprouting of the grain for longer than 1 day resulted in flours of poor cake baking quality. The cakes were low in volume, had a dip in the center, a coarse grain and a firm texture. Cookie spreads increased and cookie top grain score improved with longer times of sprouting of the grain, but the crust color of the cookies darkened. The flavor was not affected by sprouting. The thickening power of the sprout-damaged soft wheat flours decreased with time of sprouting of the soft wheat before milling into a flour. Generally, laboratory sprouting of soft white wheat had an adverse effect on the functionality of flours in soft wheat applications. Effects of field sprouting on soft wheat flour performance are expected to be similar to those observed with laboratory sprouted soft wheat.
    Type of Medium: Electronic Resource
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