ISSN:
1399-0047
Source:
Crystallography Journals Online : IUCR Backfile Archive 1948-2001
Topics:
Chemistry and Pharmacology
,
Geosciences
,
Physics
Notes:
The major endoglucanase (35 kDa) from the thermophilic fungus Thermoascus aurantiacus has been purified from culture filtrates using an affinity method and the sequence for 35 N-terminal amino acids determined. This has allowed assignment of the enzyme to subtype A6 of family 5 endoglucanases. The enzyme has been crystallized as thick plates by the hanging-drop method using ammonium sulfate as precipitant. The crystals belong to space group P212121 with cell edges a = 76.4, b = 85.7 and c = 89.5 Å, with two molecules in the asymmetric unit, and diffract to 1.62 Å resolution using synchrotron radiation. The structure will be solved by isomorphous replacement.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1107/S0907444997005404
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