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  • 1
    Electronic Resource
    Electronic Resource
    Purification and properties of a membrane-bound alcohol dehydrogenase involved in oxidation of long-chain hydrocarbons by Pseudomonas aeruginosa
    Amsterdam : Elsevier
    BBA - Enzymology 315 (1973), S. 220-232 
    Details
    ISSN: 0005-2744
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0005-2744(73)90252-0
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  • 2
    Electronic Resource
    Electronic Resource
    Separation and characterization of long-chain alcohol dehydrogenase isoenzymes from Pseudomonas aeruginosa
    Amsterdam : Elsevier
    BBA - Enzymology 276 (1972), S. 31-42 
    Details
    ISSN: 0005-2744
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0005-2744(72)90005-8
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Butyric fermentation: metabolic behaviour and production performance of Clostridium tyrobutyricum in a continuous culture with cell recycle (1990)
    Springer
    Applied microbiology and biotechnology 34 (1990), S. 172-177 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Two physiological characteristics of butyric fermentation, inhibition by the acids produced, butyrate and acetate, and dependence on the growth rate of the distribution of these acids, prompted a study of butyrate production in a continuous fermentation system with cell recycle by microfiltration. The influence of the main operating parameters, glucose input (feed concentration and dilution rate) and bleed dilution rate on production of acids and biomass was studied. The performance of the system greatly exceeded the results obtained in batch and simple continuous fermentations as a high productivity for butyrate (9.5 g l−1 h−1) was achieved whilst retaining a satisfactory concentration of butyrate (29.7 g l−1) and low acetate production (0.6 g l−1) at a cell biomass concentration of 35 g l−1. Cell growth rate was found to be a critical parameter for performance stability as oscillations in metabolic activity due to inhibition by acids were observed at bleed dilution rates below 0.016 h−1.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00166775
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  • 4
    Electronic Resource
    Electronic Resource
    Butyrate production in continuous culture of Clostridium tyrobutyricum: effect of end-product inhibition (1990)
    Springer
    Applied microbiology and biotechnology 33 (1990), S. 127-131 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Production of butyrate has been studied in continuous cultures of Clostridium tyrobutyricum. Production of acids, gases and cell biomass were determined under conditions of glucose limitation by varying either the glucose input or the dilution rate. Addition of acetate or butyrate to the cultures was also tested. The results led to the proposition that inhibition by acids acting as incouplers of energy production could provide a physiological explanation for most of the phenomena observed. It readily accounted for the higher productivities but lower product concentrations obtained in continuous culture with respect to batch or fed-batch conditions. It also explained the decrease in the ratios of butyrate to total acids and in cell yield observed at higher glucose input as well as the behaviour of the cultures under conditions of excess glucose. It could also possibly account for the partial conversion of added acetate to butyrate observed at moderate growth rates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00176512
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  • 5
    Electronic Resource
    Electronic Resource
    Conditions promoting stability of solventogenesis or culture degeneration in continuous fermentations of Clostridium acetobutylicum (1988)
    Springer
    Applied microbiology and biotechnology 29 (1988), S. 447-455 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The stability of solvent production by Clostridium acetobutylicum has been studied in continuous single-stage and two-stage fermentations. At low dilution rates, metabolic oscillations resulting from product inhibition have been observed especially in the case of fermentations controlled by product accumulation. A second type of instability also observed in product-controlled fermentations, but not in fermentations controlled by nitrogen limitation, was a long-term metabolic drift towards acid production. This acid drift has been shown to be identical to the phenomenon of culture degeneration occurring upon subculturing in batch fermentation. In addition, it was found that acid drift could be reversed by decreases in pH, temperature and dilution rate, by growth limitation in nitrogen-deficient conditions and by the addition of butyric and acetic acids. The existence of two distinct mechanisms, a short-term control (shift) and a long-term control (drift), both triggered by the same physiological conditions, is proposed in the regulation of acid and solvent production.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00269067
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  • 6
    Electronic Resource
    Electronic Resource
    The β-fructofuranosidase activities of a strain of Clostridium acetobutylicum grown on inulin (1987)
    Springer
    Applied microbiology and biotechnology 25 (1987), S. 419-425 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The β-fructofuranosidase activities of a strain of Clostridium acetobutylicum, selected for its capacity to grow on inulinic substrates, were investigated. When grown on inulin, this strain produced extracellular and intracellular β-fructofuranosidases, both of which hydrolysed inulin (inulinase activity) and sucrose (invertase activity). Inulinase activity was higher than invertase activity in the extracellular preparation, the opposite being observed for the cellular preparation. The effects of pH and temperature, substrate specificity and the kinetic constants for inulin and sucrose were studied on both preparations, as well as induction by inulin and repression by glucose and fructose of inulinase and invertase activities. The overall results were consistent with the existence of a least one inulinase, (EC 3.2.1.7), mainly but not entirely released in the extracellular medium, and an invertase (3.2.1.26) localized within the cell. Time course hydrolysis experiments of dalhia inulin and Jerusalem artichoke inulofructans by extracellular inulinase showed that this preparation had a remarkably high specificity for hydrolysis of long chain inulofructans.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00253311
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  • 7
    Electronic Resource
    Electronic Resource
    Identification and production of a rhamnolipidic biosurfactant by a Pseudomonas species (1996)
    Springer
    Applied microbiology and biotechnology 45 (1996), S. 162-168 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  A glycolipid-producing bacterium, Pseudomonas aeruginosa GL1, was isolated from the soil contaminated with polycyclic aromatic hydrocarbons (PAH) from a manufactured gas plant. The glycolipid produced was characterized in detail by chromatographic procedures as a mixture of four rhamnolipids, consisting of different associations of rhamnose and hydroxy fatty acids: the main component was monorhamnosyl di-3-hydroxydecanoic acid. The rhamnolipid composition presented marked analogies with a defined part of P. aeruginosa outer membrane lipopolysaccharides (lipopolysaccharide band A). Rhamnolipid production was stimulated under conditions of nitrogen limitation. Glycerol yielded higher productions than did hydrophobic carbon sources. Cell hydrophobicity decreased during growth on glycerol and on n-hexadecane whereas glycolipid production increased. P. aeruginosa GL1 was found to be unable to grow on a variety of 2, 3 and 4 cycle PAH. However, it was shown to persist after at least 12 subcultures in a bacterial population growing on a mixture of pure PAH, suggesting a physiological role for rhamnolipid as a means to enhance PAH availability in a mutualistic PAH-degrading bacterial community.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530050665
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  • 8
    Electronic Resource
    Electronic Resource
    Substrate availability in phenanthrene biodegradation: transfer mechanism and influence on metabolism (1995)
    Springer
    Applied microbiology and biotechnology 43 (1995), S. 952-960 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  The mechanism of phenanthrene transfer to the bacteria during biodegradation by a Pseudomonas strain was investigated using a sensitive respirometric technique (Sapromat equipment) allowing the quasi-continuous acquisition of data on oxygen consumption. Several systems of phenanthrene supply, crystalline solid and solutions in non-water-miscible solvents (silicone oil and 2,2,4,4,6,8,8-heptamethylnonane) were studied. In all cases, analysis of the kinetics of oxygen consumption demonstrated an initial phase of exponential growth with the same specific growth rate. In order to analyze the second phase of growth and phenanthrene degradation, a study of the kinetics of phenanthrene transfer to the aqueous phase was conducted by direct experimentation, with the crystal and silicone oil systems, in abiotic conditions. The data allowed the validation of a model based on phase-transfer laws, describing the variations, with substrate concentrations, of rates of phenanthrene transfer to the aqueous phase. Analysis of the biodegradation curves then showed that exponential growth ended in all cases when the rates of phenanthrene consumption reached the maximal transfer rates. Thereafter, the biodegradation rates closely obeyed, for all systems, the transfer rate values given by the model. These results unambiguously demonstrated that, in the present case, phenanthrene biodegradation required prior transfer to the aqueous phase. With the silicone oil system, which allowed high transfer and biodegradation rates, phenanthrene was directed towards higher metabolite production and lower mineralization, as shown by oxygen consumption and carbon balance determinations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530050510
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  • 9
    Electronic Resource
    Electronic Resource
    Degradation of polycyclic aromatic hydrocarbons by pure strains and by defined strain associations: inhibition phenomena and cometabolism (1995)
    Springer
    Applied microbiology and biotechnology 43 (1995), S. 156-164 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  Six bacterial strains capable of using, as sole carbon and energy source, at least one of the following polycyclic aromatic hydrocarbons (PAH), naphthalene, fluorene, phenanthrene, anthracene, fluoranthene and pyrene, were isolated. The interactions between these PAH during their biodegradation were studied in experiments involving PAH pairs, one PAH at least being used as a carbon source. All individual strains were found capable of cometabolic degradation of PAH in a range varying among strains. Inhibition phenomena, sometimes drastic, were often observed but synergistic interactions were also detected. Naphthalene was toxic to all strains not isolated on this compound. Strain associations were found efficient in relieving inhibition phenomena, including the toxic effect of naphthalene. Accumulation of water-soluble metabolites was consistently observed during PAH degradation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530050385
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  • 10
    Electronic Resource
    Electronic Resource
    Synthesis of L-tryptophan by immobilized Escherichia coli cells (1979)
    Springer
    Applied microbiology and biotechnology 7 (1979), S. 33-44 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Enzymatic production of L-tryptophan has been studied in a system containing Escherichia coli cells (induced for tryptophanase) entrapped in a polyacrylamide gel, indole and excess pyruvate and ammonia. The influence of pH, substrate concentration and temperature on L-tryptophan production, in batch and flow reactor conditions, was investigated. In flow reactor conditions, inhibition of the reaction by indole resulted in a multi steady-state pattern characteristic of substrate inhibited reactions. Any increase in flow rate at indole concentrations higher than 17 mM resulted in a dramatic decrease in the conversion rate of indole to tryptophan. A device for the practical production of L-tryptophan, which involves feeding a limiting concentration of indole to a reactor, adsorption of the synthetized tryptophan and recycling of excess pyruvate and ammonium ions was tested.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00522476
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