ISSN:
0003-276X
Keywords:
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Medicine
Notes:
The formation of a third, intermediate, layer of cells in the mouse vaginal epithelium 12 hours after estradiol administration was investigated. Making 3H-thymidine continuously available during the first 12 hours following estradiol administration to spayed mice and killing the treated animals after this period, revealed the intermediate layer of cells as constituted essentially of unlabeled cells. To the contrary, when animals were treated with estradiol 48 or 60 hours following pulse-labeling with 3H-thymidine, and killed 12 hours after hormone injection, autoradiography showed that labeled cells (which were in some part of G1 at the time estradiol was given) become a part of the intermediate cell layer. Grain count distribution in this material confirmed that they do so without undergoing further division. These results support our assumption that the formation of an intermediate layer of cells in the vaginal epithelium of spayed mice within the 12 first hours of estradiol action does not require division of basal cells under the presence of estrogen.
Additional Material:
1 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/ar.1091760408
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