ISSN:
1608-3040
Keywords:
antioxidant
;
Endomyces magnusii
;
apoptosis
;
BHT
;
DNA
;
fragmentation
;
DNA synthesis
;
ontogenesis
;
ROS
;
plant
;
protein synthesis
;
superoxide
;
wheat
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract It was found that production of superoxide (O2 – ·) is crucial for normal morphogenesis of etiolated wheat seedlings in the early stages of plant development. The development of etiolated wheat seedlings was shown to be accompanied with cyclic changes in the rate of O2 – · production both in the entire intact seedling and in its separated organs (leaf, coleoptile). First increase in the rate of O2 – · production was clearly observed in the period from two to four days of seedling development, then the rate of O2 – · production decreased to the initial level, and then it increased again for two days to a new maximum. An increase in O2 – · production in the period of the first four days of seedling development correlates with an increase in DNA and protein contents in the coleoptile. The second peak of increased rate of O2 – · production observed on the sixth or seventh day of seedling development coincides with a decrease in DNA and protein contents and apoptotic internucleosomal nuclear DNA fragmentation in the coleoptile. Incubation of seedlings in the presence of the antioxidant BHT (ionol) strongly affects their development but it does not influence the increase in DNA and protein contents for the initial four days of seedling life, and it slows down the subsequent age-dependent decrease in protein content and fully prevents the age-dependent decrease in DNA content in the coleoptile. A decrease in the O2 – · amount induced by BHT distorts the seedling development. BHT retards seedling growth, presumably by suppression of cell elongation, and it increases the life span of the coleoptile. It seems that O2 – · controls plant growth by cell elongation at the early stages of seedling development but later O2 – · controls (induces) apoptotic DNA fragmentation and protein disintegration.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1002892520658
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