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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of cutaneous pathology 21 (1994), S. 0 
    ISSN: 1600-0560
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: We carried out a cytophotometric study of the DNA content of matrix cells from normal hair follicles and those affected by androgenetic alopecia (early and established baldness), using the Feulgen technique. We thereby obtained a 2C reference value from lymphocyte nuclei from normal skin, from which we established a 2C–4C and 4C DNA content. The results obtained showed 71.75% of matrix cells in normal scalp to have a 2C content, while the remaining 28.25% was distributed between the values of 2C–4C and 4C. However, in early baldness, the number of 2C cells dropped to 48%, with a notable increase in cell populations with a 2C–4C and 4C content, which varied from 29.45% and 22.55% respectively. Finally, in established baldness, the 2C values decreased to as low as 29.56%, with the percentage of cells with a 2C–4C content reaching 38.71% and those of 4C, 31.73%.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of cutaneous pathology 20 (1993), S. 0 
    ISSN: 1600-0560
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: We carried out a quantitative study of the matrix and dermal papilla of the human hair follicle of the scalp, both normal and in various degrees of androgenetic alopecia. A stereological study showed the measured parameters to decrease with increase in the degree of alopecia, particularly as regards the total volume of the matrix and its papilla. The ratio of the two volumes increased by 30%, indicating a much more marked decrease in size of the papilla than in the matrix size. The number of cell nuclei in the matrix and the papilla of alopecic scalp was found to be 30% and 50% smaller, respectively, than those of normal scalp. Finally, a morphometric study revealed enlarged nuclei in the matrix and papilla, as shown by their increased areas, perimeters, and maximal diameters.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of cutaneous pathology 20 (1993), S. 0 
    ISSN: 1600-0560
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: We carried out a cytophotometric study of the DNA content of basal and suprabasal keratinocytes using involved and univolved histological sections of epidermis from psoriatic patients using the Feulgen technique. The reference 2C value of the DNA content was obtained from lymphocyte nuclei in a sub-epidermal infiltrate from normal skin. According to the DNA content profiles obtained for the keratinocytes of psoriatic patients, individuals were classified into three groups: Group 1, composed of individuals with a monomodal diploid profile and preferential keratinocytes with a 2C DNA content and, hence, a low proliferation activity; Group 2, consisting of patients with significantly increased 2C–4C and 4C populations; and Group 3, made up of individuals with high proportions of 4C and hyperdiploid (6C, 8C, and 〉 8C) keratinocytes. The results obtained in this work and the observed changes in the proliferative activity of the keratinocytes in the three groups of patients led us to suggest that the cytophotometric determination of the DNA content of basal and suprabasal keratinocytes may be useful as a prognostic criterion for the classification of psoriatic patients in terms of the extent of alteration of their proliferative epidermal activity.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Biomembranes 944 (1988), S. 1-12 
    ISSN: 0005-2736
    Keywords: (Sea urchin) ; Acrosome reaction ; Egg jelly ; Ion permeability ; Phospholipid composition ; Plasma membrane ; Sperm
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    BBA Section Nucleic Acids And Protein Synthesis 425 (1976), S. 492-501 
    ISSN: 0005-2787
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Mechanisms of Ageing and Development 57 (1991), S. 275-282 
    ISSN: 0047-6374
    Keywords: Cytophotometric ; Dorsal lateral geniculate nucleus ; Gallocyanin ; RNA content
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Pediatric surgery international 10 (1995), S. 105-107 
    ISSN: 1437-9813
    Keywords: Anorectal manometry ; Hirschsprung's disease ; Rectoanal inhibitory reflex (RAIR)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Anorectal manometry was performed in 32 neonates: 18 pre-term and 14 full-term infants, mean ages 6.1 and 7.1 days, respectively. The rectoanal inhibitory reflex (RAIR) was positive in 31 cases, and the relaxation waves produced by rectal distention were recorded along the anal canal and were directly related to the intensity of the stimulus. In the remaining patient the RAIR was negative due to Hirschsprung's disease. The length of the anal canal was 11.3 ± 2 mm in pre-term and 14.7 ± 2.9 mm in full-term infants (P 〈 0.001). These findings suggest that the presence of a RAIR is independent of the weight, gestational age, and birth age of the infant, and that anal canal length correlates with weight. We conclude that anorectal manometry in the neonatal period is a simple, reliable, and safe method of testing anorectal function, including the diagnosis of Hirschsprung's disease.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1435-604X
    Keywords: IR laser ; Thyroid gland ; Morphological change ; Quantitative study
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Physics , Technology
    Notes: Abstract The morphological changes produced in the thyroid glands of albino rats following radiation with a 904 nm infrared laser were studied. Two different levels of radiation were applied: 46.8 J/cm2 and 140.4 J/cm2. Evaluation of the changes in the densities of the epithelial, colloidal and follicular volumes and of the activation index revealed that the laser beam produced changes in the thyroid parenchyma. It was observed that there was a direct relationship between the severity of the lesion and the radiation energy applied.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1435-604X
    Keywords: Keywords: Infrared laser; Postnatal development; Stereology; Thyroid follicular cell; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Physics , Technology
    Notes: Abstract . A study was carried out on the secretion organelles of thyroid follicular cells during postnatal (PN) development in normal conditions and after irradiation at 46.8 J/cm2 with an infrared (IR) laser (904 nm). This was done using 11-, 21- and 35-day-old Wistar rats. The changes in the organelles were determined using stereological methods (volume and surface density) as well as electron microscopy. During normal PN development, the parameters corresponding to the different organelles of follicular cells show very high values in the 11-day-old animals, decrease in the 21-day-old animals, especially in mitochondria, then increase again in the 35-day-old animals. The results obtained show that follicular cells reach maturity early during PN development and present fluctuations in their activity during the period studied. Radiation with IR laser (46.8 J/cm2) does not seem to alter the PN development of the cells, but does induce changes in the secretion organelles, especially at day 35, among which the increase of colloid droplets and lysosomes, dilation of rough endoplasmic reticulum, and reduction of the Golgi apparatus stand out. We believe these alterations could modify thyroid hormone synthesis and secretion processes.
    Type of Medium: Electronic Resource
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