ISSN:
1432-1750
Keywords:
Lung Transplantation
;
Alveolar Lavage
;
Cytology
;
Rejection
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Alveolar lavages were performed repetitively on the normal and transplanted lungs of dogs that had received autografts or allografts without immunosuppression. One-half of the lavage returns was fixed as a cytologic smear; the other half was subjected to semi-thin section or electron microscopic examination. Of the staining methods used on the smears, the periodic acid-Schiff and Giemsa techniques were best for differentiating and counting cells. The Ladewig technique was best for evaluating the presence and location of fibrin. After autotransplantation, the proportion of so-called alveolar macrophages increased, reached a peak in 4–7 days, and then returned to normal. Phagocytized fibrin increased for the first postoperative week, but no extracellular fibrin was ever observed. After allotransplantation, a progressive decrease in the proportion, size, and vacuolization of so-called alveolar macrophages was noted along with increasing amounts of extracellular fibrin. Intracellular fibrin could only be detected up to the third day. These findings define adequate methods for preparing and staining material obtained from diagnostic alveolar lavages, and they suggest that the procedure may serve as an index of lung allograft rejection.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02101581
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