ISSN:
1574-6968
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
Four chitin synthase gene fragments were isolated from Magnaporthe grisea by use of polymerase chain reaction. A pair of degenerate primers based on conserved zymogen-type chitin synthase sequences amplified a ∼600-bp product containing three chitin synthase gene fragments. A second pair of degenerate primers, based on conserved sequences between the chitin synthases 3 of Saccharomyces cerevisiae and Candida albicans, amplified a single 770-bp fragment. The four corresponding amino acid sequences each fell into one of the chitin synthase classes I–IV, as deduced from sequence analysis. Northern analysis demonstrated that the class II gene was expressed transiently in early phases of growth, whereas the class I and III genes as well as the class IV gene were expressed throughout the entire life cycle. Furthermore, the class III gene was the most expressed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1574-6968.1998.tb13133.x
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