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  • 1
    Electronic Resource
    Electronic Resource
    Peptide and Steroid Regulation of Spermatogenesis in Primates (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 637 (1991), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1991.tb27304.x
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  • 2
    Electronic Resource
    Electronic Resource
    Severe impairment of permatogenesis in mice lacking the CREM gene (1996)
    [s.l.] : Nature Publishing Group
    Nature 380 (1996), S. 162-165 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The vector used to target the CREM gene in embryonic stem (ES) cells is shown in Fig. la. This deletion disrupts the gene and removes the coding information for both of the leucine zipper domains. Leucine zipper domains have been shown to be essential for dimerization and subsequent DNA binding ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/380162a0
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  • 3
    Electronic Resource
    Electronic Resource
    Inhibin-like and gonadotropin-like immunoreactivity in pituitary cells of male monkeys (Macaca fascicularis, Macaca mulatta) (1991)
    Springer
    Cell & tissue research 265 (1991), S. 203-209 
    Details
    ISSN: 1432-0878
    Keywords: Inhibin ; LH ; FSH ; Pituitary gland, pars anterior ; Immunocytochemistry ; Maccaca fascicularis, Macaca mulatta (Primates)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Inhibin-like immunoreactivity was detected by immunocytochemistry in the pituitaries of untreated male crab-eating macaques (cynomolgus monkey) and rhesus monkeys, in rhesus monkeys actively immunized against FSH, and in one orchidectomized crab-cating macaque. Localizations were performed by the immunogold-silver staining with 5-nm colloidal gold-conjugated second or third antibodies and by the alkaline phosphatase-anti-alkaline-phosphatase technique. Two different inhibin-specific antisera, raised against the α-subunit or the entire inhibin molecule, provided identical staining patterns. Positive label was confined to the pars distalis of the pituitary and occurred exclusively in the cytoplasm of morphologically different cell types throughout the pars distalis in all pituitaries. Staining was most prominent in clusters of chromophobic cells. The presence of inhibin-like activity in the pituitary of an orchidectomized monkey with undetectable serum inhibin levels suggests that inhibin is produced within the pituitary gland. Co-localization studies for the β-subunits of the gonadotropic hormones revealed that on average 82% of the gonadotropes were bihormonal. Using the same protocol, co-localization of inhibin-like activity with gonadotropin-like immunoreactivity revealed only a small degree of common distribution (〈15%). Inhibinpositive cells were frequently in close proximity to gonadotropic cells and, thus, paracrine effects of inhibin on gonadotropin-synthesizing cells are conceivable.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00398068
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  • 4
    Electronic Resource
    Electronic Resource
    Estimation of the duration of the cycle of the seminiferous epithelium in the non-human primate Macaca mulatta using the 5-bromodeoxyuridine technique (1997)
    Springer
    Cell & tissue research 288 (1997), S. 365-369 
    Details
    ISSN: 1432-0878
    Keywords: Key words: Spermatogenesis ; Testis ; Cell cycle ; Immunohistochemistry ; Macaca mulatta (Primates)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. A comparatively low yield of germ cells has been reported for the spermatogenic process in primates. Kinetic studies of spermatogenesis and the spermatogenic cycle are needed to investigate this phenomenon but require the application of radioactively labeled compounds or irradiation. We have therefore investigated the suitability of a non-radioactive approach, viz., administration of 5-bromodeoxyuridine, for the determination of the kinetics of the spermatogenic cycle in a non-human primate, the rhesus monkey (Macaca mulatta). Four adult in-season animals received a bolus of 33 mg/kg 5-bromodeoxyuridine, one testis from each monkey was removed 3 h later and the other testis after 10 days and 11 h. Tissue was fixed in Bouin’s solution and embedded in Paraplast. 5-Bromodeoxyuridine was localized by immunogold-silver staining with a monoclonal antibody. PAS-hematoxylin counterstaining was used for spermatogenic stage identification. At 3 h, the leptotene and zygotene spermatocytes in stages VII–IX were the most advanced 5-bromodeoxyuridine-positive cells. At 10 days 11 h, the label had advanced and pachytene spermatocytes in stages VI–IX contained 5-bromodeoxyuridine. The duration of the spermatogenic cycle was 10.42±0.07 days (range: 10.25–10.62 days). Peritubular cells and interstitial cells were rarely 5-bromodeoxyuridine-positive, and Sertoli cells were consistently negative for 5-bromodeoxyuridine. Importantly, our kinetic data closely resemble those obtained by means of the application of irradiation for this macaque species. We conclude that administration of 5-bromodeoxyuridine represents a non-radioactive reliable approach for studying kinetic aspects of the spermatogenic process in primates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004410050822
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  • 5
    Electronic Resource
    Electronic Resource
    Localization of inhibin/activin subunits in the testis of adult nonhuman primates and men (1993)
    Springer
    Cell & tissue research 273 (1993), S. 261-268 
    Details
    ISSN: 1432-0878
    Keywords: Inhibin ; Activin ; Testis ; Human ; Macaca fascicularis, Macaca mulatta, Macaca arctoides (Primates)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The localization and distribution of inhibin/activin subunits was evaluated in the testes of three nonhuman primate species (Macaca fascicularis, M. mulatta, M. arctoides), of young (31 to 43 years) and old (60 to 85 years) men, and of men with disturbed or arrested spermatogenesis using immunohistochemical techniques (peroxidase-anti-peroxidase and alkaline-phosphatase/ anti-alkaline-phosphatase technique). Specific polyclonal (anti-porcine inhibin α-1-32 and anti-bovine activin A) and monoclonal (anti-human inhibin α-1-32 and anti-human activin βA-82-114) antisera were employed. Among all nonhuman primate species and in men, inhibin/activin subunits were present in the cytoplasm of Sertoli cells and Leydig cells but not in germ cells. No relationship could be established between the staining pattern for inhibin/activin subunits and the completeness or the stage of the spermatogenic process. The staining for the βA-subunit in Sertoli cells appeared more intense in the testes of old men compared with that of young men. The majority of Leydig cells contained either the α-subunit and βA-subunit or the βA-subunit alone. The signal for the βA-subunit was remarkably intense in normal and hyperplastic human Leydig cells. These observations demonstrate the presence of inhibin/activin subunits in Sertoli cells and Leydig cells of adult primates and raise the possibility that these subunits or their respective dimers (inhibin A/activin A) might subserve a paracrine/ autocrine role in the adult primate testis. Also, the possibility of specific differences in the α-1-32 subunit and the βA-82-114 subunit region among certain primate species arises from the observation that the monoclonal antisera failed to detect the respective antigens in M. fascicularis and M. mulatta.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00312827
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