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  • 1
    Electronic Resource
    Electronic Resource
    ONE-STEP MOLECULAR HLA-DR PRESCREENING EMPLOYING A SET OF 14 SEQUENCE SPECIFIC OLIGONUCLEOTIDES IN A NON-RADIOACTIVE TETRAMETHYLAMMONIUM CHLORIDE HYBRIDIZATION PROTOCOL (1992)
    Oxford, UK : Blackwell Publishing Ltd
    International journal of immunogenetics 19 (1992), S. 0 
    Details
    ISSN: 1744-313X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: A novel non-radioactive protocol for molecular generic HLA-DR typing is introduced, employing sequence specific oligonucleotides (SSOs) enzymatically 3′-labelled with biotin-14-dATP via terminal deoxynucleotidyltransferase in a tetramethylammonium chloride hybridization procedure. The detection reaction is carried out, using streptavidine conjugated horseradish peroxidase which is bound to the SSOs, in combination with a light emitting detection system. Fourteen SSOs and one control SSO are employed for generic HLA-DR typing in a one-step protocol. In order to demonstrate the suitability of this procedure, 5 homozygous typing cell lines and samples of 11 pretyped individuals which include most serologically defined HLA-DR specificities (DR1, 2, 3, 4, 11, 12, 6, 7, 8, 9,10, 52, and DR53) are analysed with the panel of 14 SSOs. The typing results show that this protocol, which avoids the use of radioisotopes, combines high specificity and easy handling. It also allows typing of poorly amplified samples because even after longer exposition times no false positive signals were observed and is particularly suitable for routine molecular HLA-DR typing on the generic level. In addition it can easily be adapted to DP and DQ typing or DR subtyping.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1744-313X.1992.tb00082.x
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  • 2
    Electronic Resource
    Electronic Resource
    Genetic Influence on the Shaping of the Human T-Cell Receptor Repertoire: Quantitative Assessment by Competitive Polymerase Chain Reaction (1996)
    Oxford, U.K. and Cambridge, USA : Blackwell Science Ltd
    Scandinavian journal of immunology 44 (1996), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: It has been difficult to define the different factors which contribute to the shaping of the human T-cell receptor (TCR) repertoire. In this study, the influence of the polymorphic human leucocyte antigen (HLA) genes and non-HLA genes on the phenotype of the TCRBV segment repertoire was assessed in a population of HLA class I-matched individuals including three pairs of siblings. The gene expression levels of 24 TCRBV families were evaluated in the CD4+ and CD8+ T-cell subsets of unstimulated peripheral blood mononuclear cells (PBMC) by reverse transcription (RT) and a newly developed competitive polymerase chain reaction (cPCR) assay. Titration experiments demonstrated that the RT-cPCR assay was suitable for an accurate quantification of the relative TCRBV segment expression levels. The T-cell repertoires of HLA-identical siblings were found to be more similar than the repertoires of unrelated individuals. On the other hand, there was no difference in the degree of similarity between the TCRBV repertoires of CD4+ T-cells of HLA class II identical or non-identical unrelated individuals. Furthermore, although most of these individuals had identical HLA class I genes and non-identical HLA class II genes, the TCRBV repertoires of the CD4+ T cells exhibited significantly lower variabilities than the repertoires of the CD8+ T cells. The results of the RT-cPCR assay were supported by flow cytometric analysis of the CD4+ and CD8+ T-cell subsets of the same eight individuals employing 10 different TCRBV segment-specific monoclonal antibodies. These observations argue for a predominant role of non-HLA or non-polymorphic HLA determinants for the shaping of the TCRBV repertoire.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-3083.1996.d01-286.x
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  • 3
    Electronic Resource
    Electronic Resource
    A Monoclonal IgG Protein with Antibody-Like Activity for Transferrin and with X Chains of an Unusual Molecular Size (1974)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 3 (1974), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The serum of a patient with high serum iron and transferrin levels contained an abnormal brownish protein with a sedimentation coefficient of 9.9S. At pH 3.5 this protein dissociated, and on gel filtration it proved to be a complex of one molecule of monoclonal IgGI and two molecules of transferrin. At pH 7.6 the isolated 59Fe-labeled transferrin recombined with most of the separated IgGI, forming again the original complex. Of particular interest was that the polypeptide kappa chains of this IgG seemed to have a molecular size of 27,000 to 28,000 daltons. We came to the conclusion that the serum of this patient contained a monoclonal protein with antibody-like specificity for normal transferrin. Transferin only circulated as a complex with this protein at high levels in the serum and probably accumulated in the liver, giving use to severe hemosiderosis and cirrhosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1974.tb01324.x
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  • 4
    Electronic Resource
    Electronic Resource
    Age-Related Loss of Function of Alloactivated Interleukin 2-Propagated Human Primed Lymphocyte Typing Clones (1983)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 17 (1983), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Human lymphocytes alloactivated in vitro were cloned by limiting dilution in the presence of filler cells and interleukin 2 (IL 2)-containing supernatants of phytohaemagglutinin-stimulated lymphocytes. Clones with allospecific proliferative reactivity (PLT clones), measured by tritiated thymidine (3H-TdR) incorporation, were selected for extensive IL 2-dependent expansion. The cloned lines had finite lifespans, ranging from an estimated minimum of 28 to 〉 65 doublings. Function as PLT reagents, however, was retained in all cases for only an estimated 30 cell doublings. This apparent cessation of function was not caused by loss of the ability to metabolize thymidine, since lines continuing to grow for 〉 30 doublings still incorporated 3H-TdR in the presence of IL 2. An altered requirement for stimulating antigen (number of stimulating cells), or altered response kinetics, did not contribute to loss of PLT function. Exogenous IL 2 added during restimulation to responders previously ‘rested’ overnight without IL 2 did not restore the response. Thus, under present experimental conditions, functional lifespans of cloned PLT reagents appear fixed at ˜ 30 cell doublings.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1983.tb00777.x
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  • 5
    Electronic Resource
    Electronic Resource
    Typing an Unrelated Panel with PLT Cells: Association with DW Clusters (1977)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 6 (1977), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Primed LD typing (PLT) cells prepared in one laboratory (Madison) were shipped in the frozen state and tested in Tübingen on a separate panel that bad been typed with homozygous typing cells. Those PLT cells that had been grouped, on the basis of their reaction with rest cells of the Madison panel, as defining an HLA PI antigen showed identical or nearly identical patterns of reactivity with the Tübingen panel. Clear association between certain PL antigens and DW clusters as defined with homozygous typing cells could be demonstrated. Of particular interest may be combinations of certain PLT reactions with D-locus-typed cells, where the primed cells do not react as expected from the target's HLA-D type.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1977.tb02108.x
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  • 6
    Electronic Resource
    Electronic Resource
    Map Order of the Linkage Group GLO-Bf-HLA-A-PGM3 on Human Chromosome 6 (1977)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 6 (1977), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Linkage analysis of 52 families with 181 children confirms close linkage for GLO-Bf, GLO-HLA-A, and Bf-HLA-A and proves linkage between HLA-A and PGM3. Linkage for Bf-PGM3 and GLO-PGM3, respectively, seems to be likely; close linkage can be excluded. From the relative map distances, but above all from the segregation of defined linkage phases in crossover families, we can hypothesize that the sequence order of the loci should be GLO-Bf-HLA-A-PGM3.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1977.tb02102.x
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  • 7
    Electronic Resource
    Electronic Resource
    Relationship Between Fc Receptors and Ia Alloantigens: Analysis with a Sensitive Radioimmunoassay (1977)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 6 (1977), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: This paper describes the successful use of 125I-labeled IgG aggregate to detect Ia-type alloantibodies in pregnancy sera. The blockade of aggregate uptake of a variety of normal mononuclear and leukemic cell types by anti-Ia alloantibodies is analyzed. Fc receptors and Ia alloantigens are clearly two distinct molecular entities. The association between Fc receptors and Ia alloantigens on a quantitative level seems to depend on a ligand-binding mechanism to control their interaction rather than the presence of a topographical molecular tandem arrangement.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1977.tb02104.x
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  • 8
    Electronic Resource
    Electronic Resource
    Human Ia Alloantigens as Cell-Differentiation Markers of Normal and Pathologic Leukocyte Surfaces (1977)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 6 (1977), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Comparative membrane phenotypes of normal T and B lymphocytes, of normal myeloid cells, and of various leukemic cell types were established by the use of specific Ia-type alloantisera and ‘classical’ immunological surface markers, including complement receptors. 4%–8% of lymphocytes taken from mixed leukocyte cultures on day 4 showed Ia alloantigens of responder-type specificity, in addition to two known T-cell markers on the same cell surface. Together with Ia antiserum inhibition studies, this subpopulation would appear to present a human mixed leukocyte culture (MLC)-related suppressor T-cell population. Whilst such cells could not be detected in normal peripheral blood by double fluorescence, a small percentage could readily be demonstrated among cell populations from patients with systemic lupus erythematosus and rheumatoid arthritis (joint fluid). Specific Ia alloantigens were further detected on the immature myeloid cells from bone marrow but not on the more mature myeloid cell types. This establishes the Ia alloantigen system as a valuable cell surface differentiation marker in man. The appearance of complement receptors on myeloid cells was Later than that of Ia alloantigens. Ia alloantigens are expressed on various numbers of leukemic myelo- and lympho-blasts of the so-called ‘non-T, non-B’ cell types. After discontinuous Ficoll gradients to enrich specific density cell groups, mainly the Ia alloantigens and the complement receptors allow a discrimination between acute (AML) and chronic myelocytic leukemia (CML) blasts, inclucling blasts crisis cells. AML cells appear to present a less differentiated cell type than CML blasts crisis cells. For the Latter disease, a more complex picture is obtained since at least three different membrane phenotypes of blasts cells can be identified: an Ia-positive and an Ia-negative myeloid blasts and an Ia-positive lymphoid blasts. The relative quantities of these three elements can vary considerably within even a small group of patients.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1977.tb02121.x
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  • 9
    Electronic Resource
    Electronic Resource
    Detection and Quantitation of Human Ia-Type Antigens with Iodinated Protein A and Specific Purification of Antibodies Against Ia-Type Alloantigens (1977)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 6 (1977), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Antibodies directed against specific human Ia-type antigens can easily be detected and quantitated by an improved radioimmunoassay using iodinated protein A bound to a specific antibody-Ia-antigen complex on the surface of freshly drawn peripheral human leukocytes, cultured human cell lines, or lymphoid cells fixed with glutardialdehyde or formaldehyde. The same principle can also be used for the detection of Ia alloantigens on human lymphocytes when testing them with specific antisera known to contain antibodies against transplantation antigens. These anti-Ia-alloantigen antibodies had been purified by a two-step procedure involving ion-exchange chromatography on DEAE-cellulose at pH 6.3 and the specific absorption on formaldehyde-fixed Ia-alloantigen-carrying homozygous cell lines, followed by elution of these antibodies with isotonic citrate buffer at pH 3.0. In this way an about 90-fold purification could be achieved. After such a purification the highly enriched antibody fraction still reacted selectively with one specificity of the Ia antigen system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1977.tb02093.x
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  • 10
    Electronic Resource
    Electronic Resource
    Apparent Lack of Gene Dosage Effect in the PLT Assay (1977)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 6 (1977), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: In this paper the results of primed lymphocyte typing (PLT) tests are reported which show the response to HLA-D-specific stimulator and to unrelated cells. The aim was to find out the sensitivity of the PLT method for evaluating not only qualitative HLA-D-region differences but also gene dosage effects. In addition, the primed cells were restimulated with cells from HLA-D-homozygous individuals mutually negative with the specific stimulator in the usual 5-day primary mixed lymphocyte culture incubation. No gene dosage effect in PLT experiments could be observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1977.tb02116.x
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