ISSN:
1432-0991
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract DNA fromChlamydia psittaci was partially digested with Sau 3A and cloned into the lambda bacteriophage derivative vector Charon 30. From this bank about 30 of 1500 recombinants reacted with rabbit anti-C. psittaci serum. Fourteen of these clones expressed antigens varying between 15 and 76 kilodaltons (kd) as revealed by SDS-PAGE and immunoblotting. Two recombinants, expressing 27-kd and 72-kd+76-kd antigens, respectively, were further analyzed by immunoblotting with rabbit antiserum and sera from humans with different chlamydial infections. Partial restriction endonuclease cleavage of these clones showed 10 and 13 kilobases inserts, respectively.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01589384
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