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  • 1
    Electronic Resource
    Electronic Resource
    Fully Methylated oriC with Negative Superhelicity Forms an oriC-Membrane Complex before Initiation of Chromosome Replication
    Amsterdam : Elsevier
    Biochemical and Biophysical Research Communications 194 (1993), S. 1420-1426 
    Details
    ISSN: 0006-291X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1006/bbrc.1993.1983
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Evolution of an inducible penicillin-target protein in methicillin-resistant Staphylococcus aureus by gene fusion
    Amsterdam : Elsevier
    FEBS Letters 221 (1987), S. 167-171 
    Details
    ISSN: 0014-5793
    Keywords: DNA sequence ; Induction ; Methicillin resistance ; Penicillin-binding protein ; Penicillinase ; β-Lactam (Staphylococcus aureus)
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0014-5793(87)80373-3
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  • 3
    Electronic Resource
    Electronic Resource
    Induction of only limited elongation instead of filamentation by inhibition of cell division in Corynebacterium glutamicum (1998)
    Springer
    Applied microbiology and biotechnology 50 (1998), S. 227-232 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract In order to characterize the cell-division mechanism of coryneform bacteria, we tried to isolate cell-division mutants from Corynebacterium glutamicum after N-methyl-N'-nitro-N-nitrosoguanidine mutagenesis, such as Escherichia coli fts mutants, which form long filaments at the restrictive temperatures. At the non-permissive temperature, most of the mutants formed club-shaped or dumbbell-shaped, elongated rod cells, but no filament formers were isolated. Then we examined the effects of cell division inhibitors on this organism. Cephalexin and sparfloxacin, which are the inhibitors of septation and DNA synthesis respectively, and are known to cause cell filamentation in E. coli, did not cause filamentation in C. glutamicum but induced morphological changes that were similar to those observed with the temperature-sensitive ts mutants of C.␣glutamicum. These results suggest that C. glutamicum has a unique regulation mechanism, that is, the inhibition of cell division leads to cessation of cell elongation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530051281
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  • 4
    Electronic Resource
    Electronic Resource
    A murC gene from coryneform bacteria (1999)
    Springer
    Applied microbiology and biotechnology 51 (1999), S. 223-228 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The upstream flanking region of the ftsQ and ftsZ genes of Brevibacterium flavum MJ233, which belongs to the coryneform bacteria, was amplified by the inverse polymerase chain reaction method and cloned in Escherichia coli. Complementation analysis of  E. coli mutant with a defective cell-wall synthesis mechanism with the cloned fragment and its DNA sequencing indicated the presence of the murC gene, encoding UDP-N-acetylmuramate:l-alanine ligase involved in peptidoglycan synthesis, just upstream from the ftsQ gene. The B. flavum murC gene could encode a protein of 486 amino acid residues with a calculated molecular mass of 51 198 Da. A 50-kDa protein was synthesized by the B. flavummurC gene in an in vitro transcription/translation system using  E. coli S30 lysate. These results indicate that the genes responsible for cell-wall synthesis and cell division are located as a cluster in B. flavum similar to the E. coli mra region.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530051385
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  • 5
    Electronic Resource
    Electronic Resource
    Functional relationship between Escherichia coli RNase E and the CafA protein (1997)
    Springer
    Molecular genetics and genomics 253 (1997), S. 515-519 
    Details
    ISSN: 1617-4623
    Keywords: Key words RNase E ; rne ; ams ; cafA ; Escherichia coli
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We analyzed the functional relationship between the Escherichia coli RNase E and the CafA protein, which show extensive sequence similarity. The temperature-sensitive growth of the RNase E mutant strain ams1 was partially suppressed by multicopy plasmids bearing the cafA gene. Introduction of a cafA::cat mutation enhanced the temperature sensitivity of the ams1 mutant. These results suggest that there is a functional homology between these two proteins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380050352
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  • 6
    Electronic Resource
    Electronic Resource
    Probiotic culture of the rotifer Brachionus plicatilis (1998)
    Springer
    Hydrobiologia 387-388 (1998), S. 495-498 
    Details
    ISSN: 1573-5117
    Keywords: Rotifera ; Brachionus ; probiotic materials ; ecosystem ; Uchishiro organisms
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We adapted a probiotic culture system originally developed for domestic animals and fish for the intensive culture of rotifers. In this modification, a crude starter fluid was made by incubating a mixture of some 40 species of bacteria (so-called Uchishiro Microorganisms) with the by-products of other food-related materials. The probiotic culture medium (PCM) was then completed by incubation of 50 g of starter fluid (25 °C) for 3–4 days in 1 L of 50% sea water with strong aeration. After processing, the medium may be used as food for the rotifer Brachionus plicatilis. We determined the optimal levels for feeding B. plicatilis cultures by providing a starter culture of 100 rotifers different concentrations of PCM and monitoring their population growth over five days. The highest population density of B. plicatilis (351 ind ml-1) was achieved from a mixture of 20 ml PCM per 1 L rotifer water. From these experiments, we conclude that PCM is useful as food for rotifer culture if it is supplied at a satisfactory concentration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1017004124600
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    Paper (German National Licenses)
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  • 7
    Electronic Resource
    Electronic Resource
    Probiotic culture of the rotifer Brachionus plicatilis (1998)
    Springer
    Hydrobiologia 287-388 (1998), S. 495-498 
    Details
    ISSN: 1573-5117
    Keywords: Rotifera ; Brachionus ; probiotic materials ; ecosystem ; Uchishiro organisms
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We adapted a probiotic culture system originally developed for domestic animals and fish for the intensive culture of rotifers. In this modification, a crude starter fluid was made by incubating a mixture of some 40 species of bacteria (so-called Uchishiro Microorganisms) with the by-products of other food-related materials. The probiotic culture medium (PCM) was then completed by incubation of 50 g of starter fluid (25 °C) for 3–4 days in 1 L of 50% sea water with strong aeration. After processing, the medium may be used as food for the rotifer Brachionus plicatilis. We determined the optimal levels for feeding B. plicatilis cultures by providing a starter culture of 100 rotifers different concentrations of PCM and monitoring their population growth over five days. The highest population density of B. plicatilis (351 ind ml-1) was achieved from a mixture of 20 ml PCM per 1 L rotifer water. From these experiments, we conclude that PCM is useful as food for rotifer culture if it is supplied at a satisfactory concentration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1017004124600
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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